CRISPR Activation (CRISPRa) Service

Lifeasible has been deeply involved in the field of gene editing for several years, and has accumulated a wealth of experience for relevant technologies. We are committed to optimizing the CRISPRa system to help customers achieve stronger activation effects.

What Is CRISPRa Systems?

Before the birth of CRISPR technology, there was no suitable technology to activate endogenous gene expression for biological community. No matter which method is used, it is necessary to obtain the cDNA sequence of the gene in advance, and the construction cost is relatively high, especially for large-scale cDNA library, which is very difficult to produce. The emergence of CRISPR technology allows us to activate gene expression in situ.

CRISPRa 2.0

Through the modification of the CRISPR/dCas9 system, especially the modification of the guide RNA, the dCAS9 protein and the guide RNA can recognize the protein at the same time, and adsorb more transcription activators, thereby achieving a stronger transcription activation effect. The first generation of gene activation technology is about 7 times, while the second generation of gene activation technology can be as high as 40 times.

CRISPRa 3.0

The third-generation gene activation technology mainly relies on the combination of multi-gene targeting technology and gene activation technology. This gene activation technology is very effective in dicotyledonous plants， By activating the flowering genes of Arabidopsis thaliana, the flowering time of Arabidopsis can be shortened from 40 days to 13 days, realizing strong activation of biallelic genes.

Our CRISPRa Service

We are committed to optimizing the CRISPR system to carry more activation elements. After synergistic amplification, the CRISPRa system has a stronger activation effect and can really help our customers achieve gene transcription activation

We provide one-stop service, and customers only need to provide the gene information that needs to be activated. Our services cover gRNA design, vector construction, virus packaging and transfection, gRNA sequence screening, construction of CRISPRa stably transfected cell lines, target gene expression analysis, etc.

CRISPRa Structural Design

Among the many designs, the three technologies VPR, SAM and Suntag are the most popular ones.

• The three functional components (VP64, p65, RTA) in VPR technology play a synergistic role, enhancing the ability of the fusion protein to activate gene transcription.
• SAM (synergistic activation mediator) technology utilizes the ability of sgRNA to transform, adding MS2 sequence to the two neck-loop structures of sgRNA to recruit MCP protein, and MCP protein is fused with P65 and hsf1 domains.
• Suntag technology uses the principle of antigen and antibody. The long-chain antigen fused to dCas9 can recruit VP64 carried by multiple antibody scFv at a time, amplifying its activation effect

CRISPRa Experiment Procedure

We hope to share our technology and results with customers to help customers advance their projects better and faster. For inquiries, please contact us.

Why Choose Lifeasible?