Daucus Carota Transformation

Daucus carota, also known as carrot, is a plant of the Umbelliferae family. Carrot is one of the most important vegetable crops having nutritious tubers rich in carotenoids. Carotene is the main source of vitamin A, which can promote growth and prevent bacterial infections, as well as having the function of protecting epidermal and epithelial tissues. In addition, carrots contain quercetin which can increase coronary blood flow, promote the synthesis of adrenaline, lower blood pressure, and have anti-inflammatory effects. Research on carrots has made very impressive achievements in identifying nutritional components, genetic engineering to improve new varieties, and genome structure. However, the acquisition of genetic material in carrots relies mainly on natural mutants, severely limiting the study of functional genomics in carrots. The research on genetic transformation can not only promote the development of basic carrot research but also lay the foundation for the molecular improvement of carrot genetic traits.

What We Offer

Lifeasible provides one-stop personalized customization services for customers, including experimental design, vector construction, plasmid transformation, positive graft screening, PCR identification, and positive seedling transplantation guidance. To suit the different customers' different purposes, we offer a wide range of Agrobacterium strains (GV3111, LBA4404, GV3101, etc.), as well as custom vectors with variant selectable markers (kanamycin, Hygromycin, Phosphinothricin, G418, etc.) for your use. Our carrot genetic transformation service uses carrot seedlings as transgenic recipient material, and after Agrobacterium infestation, differentiation, screening, and identification, we finally obtain positive seedlings. Our experimental steps are operated by a team of experienced experts, maintaining strict QC standards. We offer the following vector construction services for you to choose from.

Service Flow

1. Sample submission requirements

Customers can provide constructed carrot transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

During the experimental process, we keep our customers informed at key points in the experiment. We also proactively report on the progress of the experiments on a regular basis, and you can contact us at any time to get updates on the results of your experiments. The advantage of our one-stop-shop service is the maximum simplification of your carrot genetic modification research project. All you need to do is submit samples and clarify transformation requirements with us.

3. Daucus carota transformation results in delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We have a professional technical team with years of experience in carrot genetic transformation, which can achieve great ensure the high efficiency and success rate of the experiment.
• We provide one-stop, customized service to achieve the maximum simplification of your research project to save you precious time.
• We offer a wide range of Agrobacterium strains and commercial and custom vectors with various mutation selection markers, giving you a wide range of options.
• Our versatile carrot transformation platform is based on the mainstream Agrobacterium infestation method, ensuring professional and reliable results.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in daucus carota transformation. We can provide customized solutions to help you study a wide range of carrot varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.