Eremochloa ophiuroides Transformation

Eremochloa ophiuroides is a Poaceae perennial herbaceous plant, that belongs to the warm-season dwarf turfgrass, Eremochloa ophiuroides is one of the world's top three warm-season turfgrasses, is also an excellent soil consolidation and slope protection materials, widely used in roads, embankments, slopes and other landscaping. It is also one of the world's three warm-season grasses and is an excellent soil-solidifying slope protection material, widely used in greening roads, embankments, and slopes. In addition, Eremochloa ophiuroides also has certain medicinal value. For example, it can be used to treat abdominal pain after heatstroke. Eremochloa ophiuroides is often attacked by unfavorable environments, which can cause certain stress or even injury to the growth and development of plants and bring significant losses to agricultural production, so it is necessary to carry out genetic transformation studies on Eremochloa ophiuroides to select resistant plants. Genetic transformation research to select new varieties with increased resistance is significant for Eremochloa ophiuroides breeding and application.

Based on our advanced technology platform and experienced research team, Lifeasible is committed to providing comprehensive and efficient plant genetic transformation technology services to meet the research needs of a wide range of researchers on various plants. Our Eremochloa ophiuroides genetic transformation services have helped researchers to effectively promote Eremochloa ophiuroides research and provide new germplasm materials and a promising technology platform for further expansion of turf planting areas.

What do we offer?

We used Agrobacterium tumefaciens and gene gun transformation method to carry out genetic transformation research on Eremochloa ophiuroide. We transferred the target genes related to the regulation of plant resistance into the healing tissues of Eremochloa ophiuroides, and regenerated plant obtained through the screening of healing tissues, differentiation, and rooting process, and positive plants were obtained by molecular testing. In addition, we continuously optimized the transformation method during the transformation process to provide reference and information for the transformation of other turfgrasses and biotechnology-improved varieties.

• Agrobacterium-mediated transgenic method of Eremochloa ophiuroides for transferring target genes. We conducted Agrobacterium-mediated transgenic experiments using mature embryonic healing tissues of Eremochloa ophiuroides as the receptor, and the healing tissues obtained by transformation were positively identified after the regenerated seedlings were obtained in culture.　
• Optimization of Agrobacterium method for transforming Eremochloa ophiuroides using GUS transient expression We performed GUS staining of transformed healing tissues to detect transient expression of genes. The optimized Agrobacterium-mediated transgenic method for Eremochloa ophiuroides was obtained by designing different treatments using healing tissues with varying times of incubation, hypertonic medium, AS pre-culture, and ultrasound-assisted infiltration using GUS staining for transient expression.
• Transfection of target genes into the Eremochloa ophiuroides genome using the gene gun method. We used mature embryonic healing tissues of Eremochloa ophiuroides as the recipient for transformation by the gene gun method, and the healing tissues obtained from the transformation were cultured to obtain regenerated plants, which could be screened by PCR to see if positive plants were obtained.

Why choose us?

The characteristics of Poaceae plants make the tissue culture and regeneration system of many turfgrasses and forages imperfect, and the genetic transformation rate is low. Therefore, most of the transgenic researches on turfgrasses are in the stage of establishing and perfecting the genetic transformation system, and relatively few researches on transgenic operation and application have been carried out. After years of research, we have established a perfect genetic transformation platform for turfgrass. We provided perfect genetic transformation services for Eremochloa ophiuroides by optimizing the transformation conditions and establishing an efficient regeneration and transformation system.

Lifeasible has established an Agrobacterium-mediated genetic transformation system for Eremochloa ophiuroides and obtained the best transformation efficiency by optimizing the combination of various transformation conditions. This service is essential for the genetic engineering of Eremochloa ophiuroides and lays the foundation for obtaining high-quality new Eremochloa ophiuroides lines. If you are interested in us, please feel free to contact us.