Hordeum vulgare Transformation

Hordeum vulgare is an important food crop. Currently, the main means of Hordeum vulgare selection is still traditional breeding, which is time-consuming and requires a lot of human and material resources. With the rapid development of molecular biology technology, transgenic technology has been recognized as one of the effective techniques to innovate crop germplasm and select new varieties. Establishing and optimizing the Agrobacterium-mediated genetic transformation system for Hordeum vulgare provides a reliable solution for variety selection and promotion of its research and application. Lifeasible has rich experience in the research and development of plant genetic transformation systems, and we are committed to establishing the most comprehensive plant genetic transformation technology platform. After years of research, we have successfully established the Hordeum vulgare genetic transformation system using Agrobacterium-mediated methods and helped researchers to further promote the development of Hordeum vulgare transgenic research.

What do we offer?

We used young and mature embryos as explants and compared the effects of different embryo treatments, different basal media, different carbon sources, and different hormone ratios on the induction and differentiation of healing wounds to screen and determine the suitable receptor materials for the genetic transformation of Hordeum vulgare, and to derive the optimal transformation conditions. In addition, we compared the effects of different genotypes on the healing and differentiation rates based on the optimized parameters to broaden the receptor genotypes of Hordeum vulgare genetic transformation and lay the foundation for establishing a further barley genetic transformation system.

Based on the establishment and optimization of the regeneration system of Hordeum vulgare mature embryo, we introduced the β-glucuronidase gene (i.e., GUS gene) into the healing tissues of Hordeum vulgare mature embryo by Agrobacterium-mediated method and optimized the Agrobacterium-mediated genetic transformation system of Hordeum vulgare through the parameter screening. The Hordeum vulgare genetic transformation system we established, using Hordeum vulgare seeds as transformation recipients, could successfully obtain transgenic Hordeum vulgare-positive materials and cultivate Hordeum vulgare transgenic plants by inoculation of prickly embryos with Agrobacterium rhizogenes.

Notice to customers

• Customers can directly provide the constructed transformation vector or entrust our company to carry out vector construction.
• By default, we adopt specific Hordeum vulgare receptor species, and if the collaborator wants to transform a specific mutant species, the two sides will negotiate separately.
• Due to various factors, the cost of experiments may be adjusted from time to time; please inquire directly for the specific cost.
• We can carry out transgenic Hordeum vulgare technology services all year round.
• Customers can make personalized requests for project service options and delivery targets, and both parties can negotiate to determine the technical solutions and fees.

What do we offer?

• A description of the progress of the experimental project.
• Sequencing report and pictures of vector construction.
• Pictures of explant invasion, induced healing, embryonic healing, tissue differentiation, and plant regeneration.
• Gel electrophoresis diagram of PCR detection of target gene in T0 generation regenerated plants.
• T0 generation Hordeum vulgare transgenic seedlings, 10-20 T0 generation positive seedlings for each transformation experiment.

Why choose us?

• Full technical support to help customers to help plant scientific research.
• Customized experimental program, innovative experimental design, to meet customers' different genetic transformation needs.
• Strict quality control system, and standardized transformation process to ensure the success rate of plant transformation.

Lifeasible specializes in technology development and experimental services in molecular biology and plant genetic engineering, and we provide comprehensive technical support for research institutions and seed companies. We are characterized by our efficient and abundant plant genetic transformation system. If you are interested in us, please feel free to contact us.