Improved Saccharification Efficiency

Low glycosylation efficiency leads to a high cost of enzymatic glycosylation. Improving glycosylation efficiency is important for improving biofuel production and high value-added chemical production and has shown important applications in industrial production.

Lifeasible is deeply involved in agricultural synthetic biology research, aiming to advance the progress of agricultural synthetic biology research by providing advanced technology services and developing innovative solutions. We provide genetic engineering technologies that can now be effectively used to improve the efficiency of plant glycation and thus generate greater economic benefits.

We offer various technologies to help improve the efficiency of plant saccharification effectively

• TALEN (transcription activator-like (TAL) effector nucleases)

TALEN technology provides an important technological basis for synthetic biology in agriculture, where we can construct recombinant nucleases that target any specific nucleic acid target sequence, interrupt the target gene DNA at a specific site, and then perform DNA manipulation. We have used TALEN technology extensively in our research on improving glycation efficiency. For example, we have effectively achieved improved saccharification efficiency in biofuel production through the TALEN-mediated mutation of multiple caffeic acid O-methyltransferase alleles in sugarcane.

• Antisense gene technology

Antisense technology is widely used in agricultural breeding research, and we can breed plants with special functions through targeted mutations of genes through antisense technology. The antisense technology we offer will play an important role in agricultural development. For example, we can breed transgenic antisense coffee acyl coenzyme A3-0-methyltransferase (CCoAOMT) gene plants using antisense gene technology and found that transgenic plants with suppressed CCoAOMT gene expression have significantly higher glycation efficiency, which can significantly improve glycation efficiency and increase glycation yield in biofuel production.

• Gene expression regulation

We can effectively improve the glycation efficiency of plants by regulating certain specific genes, such as making specific genes functionally absent or down-regulated. This approach we have successfully used in rice, Arabidopsis, poplar, eucalyptus, pine, and other plants and has yielded a large economic value.

Our service processes

Service advantages

• Higher cutting efficiency. Optimized screening of the N-terminal and C-terminal of TALEN vectors, optimized for higher cutting efficiency of TALEN.
• Greater specificity. By optimizing the length and module selection of TALEN recognition modules, the specificity and efficiency of TALEN are continuously improved.
• Broader targeting range. Unlock the traditional restriction that the first recognition base of TALEN is T, and allow arbitrary selection of the starting base.

Lifeasible is committed to providing advanced technical support for agricultural synthetic biology and effective solutions to help solve agricultural development challenges. Our advanced genetic engineering technologies play a critical role in driving sustainable development in modern agriculture. With our years of service experience and advanced genetic engineering technologies, we aim to provide high-quality, efficient, one-stop service for agricultural synthetic biology. If you are interested in us, please feel free to contact us.