Lotus corniculatus Transformation

Lotus corniculatus is a perennial herbaceous plant of the genus Lotus corniculatus in the family of Leguminosae, which is native to the temperate regions of Eurasia and now distributed in Europe, North America, Australia and New Zealand. It is widely used for orchard grass, green manure, pasture improvement, and forage. It has good medicinal, economic, and ornamental value. Currently, the breeding cycle of conventional breeding methods is long and inefficient. Therefore, advanced molecular biology-related techniques and tools can promote the research and quality cultivation of Lotus corniculatus.

Lifeasible has extensive experience in plant genetic transformation. We use the Agrobacterium-mediated method to realize Lotus corniculatus transformation. Customers can provide plasmids, Agrobacterium, etc., and we complete the whole experimental process, from constructing vectors to obtaining transgenic seedlings for you. The Lotus corniculatus genetic transformation service we provide lays the foundation for its transgenic research.

What do we offer?

Establishing a genetic transformation system for Lotus corniculatus is the basis for improving its quality and expression of exogenous proteins using genetic engineering. Currently, we obtain a high frequency of regenerated plants mainly from Lotus corniculatus anthers, meristematic tissues, somatic embryos formed by healing, protoplasts, or roots. The transformation systems we utilize are primarily mediated by Agrobacterium, with Agrobacterium rhizogenes mediating the highest rate of genetic transformation of Lotus corniculatus. In addition, we have experimentally investigated the efficiency of different infestation methods to improve Lotus corniculatus transformation, thus determining the optimal transformation conditions to ensure the improvement of its transformation success.

Our services of Lotus corniculatus transformation

• Vector construction.
• Lotus corniculatus recipient healing tissue induction and succession.
• Agrobacterium-mediated Lotus corniculatus genetic transformation, screening.
• Differentiation of positive healing tissue into seedlings.
• Rooting and strengthening of regenerated green seedlings.
• PCR detection of exogenous genes in T0 generation transgenic seedlings.

Our service workflow

Experimental results we provide

• Pictures of some samples of genetic transformation and screening.
• Pictures of some samples of differentiation and regeneration of healing tissue.
• Agarose gel electrophoresis of PCR detection of exogenous gene in T0 generation transgenic seedlings.
• T0 generation transgenic seedlings, 10-20 T0 generation positive seedlings for each transformation experiment.

Our Technical Advantages

• Efficient experimental process. We have developed specific transformation methods for Lotus corniculatus genetic transformation, which can maximize the success rate of experiments.
• Mature genetic transformation system. After years of experimental research, we have determined the optimal transformation conditions to ensure the smooth progress of the experiment and help customers save a lot of research time.
• Professional research team. We are equipped with advanced experimental instruments and equipment, and our experienced research team provides you with professional and high-quality biotechnological support.

Lifeasible is at the leading level of the industry in plant genetic transformation research. We provide plant genetic transformation services, and we have established pleasant cooperation with many universities and research institutes with a strong plant transformation platform. We use Lotus corniculatus healing tissue as the receptor to develop an efficient Lotus corniculatus genetic transformation system, which lays the foundation for future Lotus corniculatus genetic improvement. If you are interested in us, please feel free to contact us.