Momordica charantia Transformation

Momordica charantia is an annual climbing herb, native to Indonesia, and now cultivated all over the world, Momordica charantia has good medicinal value, food value, ornamental value, etc. For example, Momordica charantia juice has a good hypoglycemic effect. Momordica charantia has good medicinal value, edible value, ornamental value, etc. For example, Momordica charantia juice has good hypoglycemic effect, and Momordica charantia vine has decorative value, and urban residents with courtyard are often planted on their initiative. Promoting the genetic transformation of Momordica charantia will have a positive impact on the utilization of Momordica charantia genetic resources and the cultivation of high-quality varieties. Lifeasible is committed to plant genetic transformation, and we have advanced transformation platforms and professional knowledge of gene transformation. We are always at the forefront of plant genetic transformation technology, aiming to provide efficient and reliable Momordica charantia genetic transformation technical support services.

What do we offer?

• Selection and preparation of explants. We need to select suitable exosomes, such as hypocotyls or petiole segments of Momordica charantia seedlings. And sterilize the exosomes to reduce the risk of microbial contamination.
• Establishment of the genetic transformation system. In the establishment of the Momordica charantia genetic transformation system, we utilized Agrobacterium-mediated transformation or bioballistic particle delivery (biolistic particle delivery) technology to deliver vectors containing the target genes into Momordica charantia cells via Agrobacterium or gold particles.
• Post-transformation processing. This process involves placing the transformed explants in a suitable medium that provides the necessary nutrients and hormones to promote cell proliferation and regeneration. Screening is done based on the selective agents used to ensure that only transformed cells can grow.
• Regeneration and characterization. Transformed cells were regenerated under suitable conditions to form healing tissues, which differentiated into regenerated plants. Subsequently, we detected and confirmed the integration and expression of exogenous genes by molecular biology methods (e.g., PCR, Southern hybridization).
• Culture and analysis of transgenic plants. We transferred the regenerated transgenic plants to a greenhouse or a suitable growth environment for further culture. The transgenic plants were also analyzed phenotypically, physiologically, and biochemically to assess the performance of the improved traits.

Our experimental procedure

Momordica charantia transformation technology platform

Our research objectives for Momordica charantia transformation

• Yield enhancement. Yield improvement of Momordica charantia through genetic transformation techniques to make it more adaptable to different growing environments and conditions.
• Quality improvement. Improvement of the nutritional composition of Momordica charantia fruits, such as increasing their content of vitamins, minerals and bioactive components to enhance the food value.
• Fruit trait improvement. Improve Momordica charantia fruit traits such as shape, size, color, etc., to enhance its marketability and consumer acceptance.
• Disease resistance enhancement. Enhance Momordica charantia's resistance to pests and diseases and reduce the use of pesticides by transforming disease resistance-related genes.
• Stress tolerance enhancement. Improve Momordica charantia's ability to tolerate adversities such as drought, salinity and low temperature to expand its cultivation range.

Why choose us?

• Advanced services. We are always at the forefront of plant genetic transformation research and constantly optimize the transformation system to ensure the provision of high-quality genetic transformation technology services.
• High success rate. Our experienced team of experts has been committed to the development of plant genetic transformation systems for a long time, which significantly improves the success rate of experiments.
• Efficient experimental process. We can carry out genetic transformation projects at any time of the year to ensure that the transformation results are submitted in the shortest possible time, which helps our customers save research time.

Lifeasible has a solid theoretical foundation and strong practical technical support in plant genetics and technology. We are committed to providing one-stop technical services for plant gene function research and application. If you are interested in us, please feel free to contact us.