Petunia Hybrida Transformation

Petunia hybrida is a perennial herb in the Solanaceae family. It has long been widely used in gardens worldwide because of its rich flower color and adaptability. Moreover, due to its short life cycle, clear genetic background, and easy gene introduction mediated by Agrobacterium, petunia has become a model plant for floral molecular biology research, providing favorable conditions for verifying gene functions and creating new varieties of plant genetic engineering. Currently, the genetic transformation system of Petunia has been studied in greater depth. Lifeasible has been working on a commercial petunia genetic transformation system and promoting the usage of genetic engineering in petunia breeding improvement.

What We Offer

Lifeasible has a proven and stable petunia gene transformation platform that enables efficient and professional genetic transformation services. Our petunia genetic transformation is not restricted by variety or season and can be experimented with at any time of the year. We have comprehensive knowledge and experienced experts in designing and operating genetic transformation experiments in petunia, mainly focusing on the Agrobacterium infestation method. Firstly, we use petunia seedlings as transgenic recipient material. Then, we use Agrobacterium tumefaciens carrying the target gene vector to infest the seedlings and insert the T-DNA into the genome. Finally, after healing tissue differentiation and resistance screening, we will have positive transgenic plants that meet your needs. We have the following gene vector construction services for you to choose from.

Service Flow

1. Sample submission requirements

Customers can provide constructed petunia transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

The whole process of our experiments is open and transparent. Not only will we keep you informed at key points of the experiment, but you can also contact us at any time for immediate experimental progress. Our one-stop personalized, and customized solution aims to simplify your petunia transgenic research to the greatest extent possible, and you will feel relax and comfortable working with us. All you need to do is submit your samples as required, clearly communicate your needs to our experts, and then leave the rest to us.

3. Petunia hybrida transformation results delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We provide our customers with an expandable and multifunctional petunia transformation platform, enabling customization of experimental protocols and experimental procedures, covering experimental design, vector construction, plasmid transformation, positive graft screening, etc.
• We have an optimized seedling transformation system that is not limited by variety or season. For popular petunia species, we have a good supply of sterile seedlings all year round to facilitate genetic transformation programs at any time.
• We have a professional technical team that has been engaged in the genetic transformation of petunia for many years, which can guarantee the success rate and high efficiency of the experiment.
• Our genetic transformation platform is based on the mainstream Agrobacterium transformation method and is suitable for multiple resistance screening with the advantages of high efficiency, stability, and single-copy insertion.

Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in petunia hybrida transformation. We can provide customized solutions to help you study a wide range of petunia varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.