Pollen Viability and Germination Analysis

Pollen viability refers to the ability of pollen grains to survive and function under specific conditions, while germinability refers to the ability of pollen grains to germinate and fertilize when they encounter a suitable environment successfully. Both are essential factors in assessing the potential for pollen distribution in the field. High-quality pollen is critical to pollination success and determines fruit quality. Pollen viability and germination analyses are critical to ensuring uniformity of fruit size and yield in fruit tree cultivation. Lifeasible specializes in pollen viability and germination analysis services, and we aim to help fruit growers and agribusinesses optimize the pollination process to improve fruit yield and quality.

Importance of Pollen Viability and Germination Analysis

• Improve pollination success. By testing pollen's viability and germination power, the best quality pollen can be screened for pollination, thus improving the success rate of pollination.
• Optimize resource allocation. Knowing the quality of pollen can help fruit growers and agribusinesses allocate resources more efficiently, for example, by rationalizing the allocation of human and material resources during the pollination season.
• Improve fruit quality. Good quality pollen ensures accurate genetic information transmission, improving fruit quality and market competitiveness.
• Environmental adaptation assessment. Pollen viability and germination power are also important indicators for assessing the adaptability of plants to environmental changes.

Our Service for Pollen Viability and Germination Analysis

We use advanced laboratory techniques with established protocols for pollen grain viability and germination analysis. These methods include, but are not limited to, those shown below.

• Viability assay. The physiological status of pollen grains is assessed by staining methods and microscopic observation. Commonly used stains include tetrazolium salt (MTT) and Evans blue, which effectively differentiate between active and inactive pollen.
• Germination capacity assay. The pollen germination was observed in a controlled laboratory environment using culture medium and appropriate temperature and humidity conditions. The germination rate and germination time are recorded to obtain accurate data.
• Multifactorial analysis. A comprehensive analysis combining environmental factors, such as temperature, humidity, and pollen characteristics, to help fruit growers understand differences in pollen performance under different conditions.

Our Process for Determination of Seed Germination

• Sampling. Collect normal flowers that are fully ripe and have cracked anthers with pollen dispersal.
• Preparation of culture medium. The prepared medium was poured into Petri dishes and used after cooling.
• Sowing. Solid medium, liquid medium for sowing.
• Cultivation. After several hours of incubation at a specific temperature and microscopic examination, the total number of pollen grains and the number of germinated grains were counted, and the germination rate was calculated.

Fig.2 Process of seed germination determination. (Lifeasible)

Application of Pollen Viability and Germination Analysis

• Fruit tree cultivation. Provides a scientific basis for pollinating cherry, peach, almond, kiwi and other fruit trees.
• Fruit tree pollination. Ensure that pollen used for pollination has high viability and germination power to improve pollination success.
• Genetic research. Evaluating pollen viability and germination power in genetic breeding studies of fruit trees can provide data to support the improvement of fruit tree varieties.

Why Choose Us?

• Experienced. We have extensive service experience in pollen viability and supporting fruit tree pollination decisions.
• Advanced platform. We have established a robust technical platform for pollen viability and germination force analysis to support pollination research better.
• Customized services. We can provide customized pollen viability and germination analysis services according to the relevant requirements of researchers and agribusinesses.
• Team of experts. Our experts and technicians have extensive knowledge in pollen characterization and fruit tree pollination research.

Fig.3 Advantages of our services. (Lifeasible)

Lifeasible is a specialized biotechnology company aiming to provide comprehensive biotechnological support for agricultural research. We provide pollen viability and germination analysis services to help agricultural researchers and practitioners achieve more efficient and sustainable agricultural production. If you are interested, please feel free to contact us.