Polygonatum sibiricum Transformation

Polygonatum sibiricum is a perennial herb. It has good food value, and the fleshy rhizome can be eaten raw with a sweet flavor. In addition, Polygonatum sibiricum has good medicinal value and can be used to relieve various symptoms such as weak spleen and stomach, tiredness, and fatigue. Genetic transformation research on Polygonatum sibiricum is one of the essential means to explore Polygonatum sibiricum to regulate important agronomic traits and improve traits. Therefore, it is of excellent research significance to conduct genetic transformation studies on Polygonatum sibiricum.

Lifeasible is a comprehensive plant research company which has been recognized by many researchers for its plant transformation services. We provide one-stop plant transformation services, including experimental design, vector construction, plasmid transformation, screening, and testing of positive plants. Based on our advanced and complete technology platform and experienced expert team, we have established the Polygonatum sibiricum genetic transformation technology platform.

What do we offer?

• Select high-quality Polygonatum sibiricum germplasm resources, such as high-product lines or specialty varieties. For example, we can select Polygonatum cyrtonema for salt stress research.
• Establishment of tissue culture system of Polygonatum sibiricum, including healing tissue culture, shoot tip culture, etc., and obtaining a large number of sterile plants. Establishing the tissue culture system of Polygonatum sibiricum lays the foundation for genetic improvement and industrial application of Polygonatum sibiricum.
• Using Agrobacterium-mediated genetic transformation technology, target genes, such as some genes related to metabolism and stress resistance, were introduced into Polygonatum sibiricum cells. In the genetic transformation of Polygonatum sibiricum, we first need to construct a recombinant plasmid containing the target genes, which will be inserted into the T-DNA region and include the T-DNA boundary sequences, replication origins, mutant promoter fragments, reporter genes, and resistance genes. The recombinant plasmid is then introduced into Agrobacterium receptor cells by electrotransformation. Next, Agrobacterium containing the recombinant plasmid was used to infiltrate the root incision of Polygonatum sibiricum, and the target genes were integrated into the genome of Polygonatum sibiricum cells by activating the transfer of T-DNA through the trans-activation of the expressed protein in the Vir region of the auxin plasmid with the T-DNA region of the recombinant plasmid. With the formation of healing tissues, the newborn cells all contain the gene of this target fragment, and the transformed plants are obtained by cell and tissue culture techniques.
• In positive plant identification, we can utilize genomic DNA extraction and PCR amplification to extract genomic DNA from wild-type (WT) and transformed Polygonatum sibiricum root lines to make specific upstream and downstream primers for PCR amplification to identify the positive root lines. In addition, we can utilize GUS staining assay and GFP fluorescent protein identification to identify transgenic-positive plants.

Our service process

Our research objectives for Polygonatum sibiricum transformation

• Establishment of an efficient transformation system. By optimizing the transformation conditions of Polygonatum sibiricum, we were able to ensure the efficient and stable expression of exogenous genes.
• Gene function validation. Our functional validation of specific genes in Polygonatum sibiricum can reveal the roles of these genes in the growth and development, metabolic pathway regulation, etc., of Polygonatum sibiricum.
• Biosynthetic pathway studies of medicinal components. We utilize genetic transformation technology to study the biosynthetic pathways of critical medicinal components such as polysaccharides and saponins in Polygonatum sibiricum, which will provide a molecular basis for improving the content of medicinal components.
• Germplasm resource innovation and new variety cultivation. We use genetic transformation technology to improve Polygonatum sibiricum traits, such as disease resistance and adaptability, and to breed superior varieties.

Notice to customers

• Experiment fee. The experimental fees we charge are affected by various factors, please consult us directly for specific fees.
• Experiment time. We usually need 4-6 months, but the specific time may be affected by weather factors and other factors.
• Experiment vectors. Customers can provide the target vector, or we can be commissioned to carry out vector construction to ensure the success rate of the experiment.

Why choose us?

• Experienced. We have been developing plant regeneration and genetic transformation systems for a long time, and we have mature and stable plant genetic transformation technology, which can guarantee the success rate of the experiment.
• Efficient experimental process. We constantly optimize the genetic transformation system and can carry out genetic transformation projects at any time, and our genetic transformation service is not limited by the season.
• Advanced technology platform. We provide an Agrobacterium transformation technology platform that can quickly, efficiently, and stably transform plant target genes.

Lifeasible has independently developed a genetic transformation system for Polygonatum sibiricum after years of development and technological research, which provides certain references for the screening of critical genetic resources and breeding of Polygonatum sibiricum. It can provide stable Polygonatum sibiricum transgenic technology services for most researchers. If you are interested in us, please feel free to contact us.