Rhododendron lapponicum Transformation

Rhododendron lapponicum is a deciduous shrub, is one of the world's four central alpine flowers, is an essential species of forest vegetation, widely distributed in Europe, Asia, and North America, mainly produced in East Asia and Southeast Asia, has a high ornamental value and a certain degree of medicinal value, in recent years in the flower market is very popular. Rhododendron lapponicum is cold-tolerant, but its acidophilic and salinity characteristics are the most restrictive factors for cultivation and utilization. Rhododendron lapponicum is hardy, but its acidophilic and salt avoidance characteristics are the most significant limiting factors for cultivation and utilization. Genetic transformation of Rhododendron lapponicum has been successfully used in the research and cultivation application of Rhododendron lapponicum.

Lifeasible is committed to the study of plant genetic transformation, we use Rhododendron lapponicum leaves as explants, using genetic engineering means to transfer the target gene base into Rhododendron lapponicum, and its genetic transformation system was systematically investigated, to obtain the increased resistance and ornamental value of the Rhododendron lapponicum transformation lines. Based on the genetic transformation of Rhododendron lapponicum, we developed a stable and efficient genetic transformation method to fill the technological gap and provide reliable technical support to promote the breeding of Rhododendron lapponicum.

What do we offer?

We used Rhododendron lapponicum healing tissues as the recipient material and introduced the target gene into the Rhododendron lapponicum genome using the Agrobacterium-mediated method. Meanwhile, we did further research on several factors affecting the transformation efficiency of Rhododendron lapponicum to establish the Rhododendron lapponicum genetic transformation system. Then RT-PCR test was performed on resistant plants to prove that the target gene was successfully transferred into Rhododendron lapponicum. Our main experimental procedure is as follows.　

• The Rhododendron lapponicum shoot regeneration system was established with sterile seedling leaves as explants.
• We investigated the effects of pre-culture time, bacterial solution concentration, infestation time, and co-culture time on Agrobacterium-mediated transformation by studying Agrobacterium-mediated genetic transformation to optimize the transformation conditions and improve the transformation efficiency.
• Using the optimized transformation receptor system, the target gene was transferred into Rhododendron lapponicum genome, and resistant plants were obtained.
• The RT-PCR assay proved positive plant strains by successfully transferring the target into Rhododendron lapponicum genome.

Based on our advanced technology platform and experienced expert team, we have established an efficient and reliable Rhododendron lapponicum transformation system mediated by Agrobacterium, which has the advantages of a short transformation cycle, simple operation, low cost, and high transformation efficiency, with a transformation cycle of 50-60 days.

Our research objectives of Rhododendron lapponicum transformation

• Shortening the breeding cycle. We can shorten the breeding cycle by obtaining genetically transformed plants in a shorter time through Agrobacterium-mediated whole-plant infection method. This can significantly accelerate the breeding process of new varieties of woody perennials such as Rhododendron lapponicum.
• Improvement of ornamental traits. Rhododendron lapponicum is famous for its beautiful plant shape and brilliant flower color, and the genetic transformation technology can be used to improve these ornamental traits, such as flower color and shape, to meet the demand of gardening and the annual flower market.
• Improvement of environmental adaptability. We can improve the adaptability of Rhododendron lapponicum to unfavorable environmental conditions, such as drought resistance, cold resistance, disease resistance, etc., through genetic transformation technology, to expand its cultivation range and application scenarios.

What do we deliver?

• A certain number of transformed plants.
• Detailed DNA sequence identification report.
• Information about test primers, vector sequences, mutated genes, etc.
• Complete, detailed and comprehensive experimental data and reports.
• Experimental data and pictures.

Why choose us?

• Professional technical team. We have a professional team composed of experts in genetics, molecular biology, plant physiology, etc., which can provide professional genetic transformation services.
• Efficient transformation system. We have developed an efficient genetic transformation system for Rhododendron lapponicum, such as the Agrobacterium-mediated whole-plant infection method for alpine Rhododendron lapponicum, which is characterized by short transformation cycle, simple operation, low cost, and high transformation efficiency.
• Rich practical experience. We have rich practical experience in the genetic transformation of Rhododendron lapponicum, and can develop appropriate transformation strategies according to the characteristics of Rhododendron lapponicum.
• Innovative R&D capability. We have strong R&D capabilities to optimize the transformation process, improve the transformation efficiency, and develop new transformation methods to meet the ever-changing market needs.

Our service workflow

Lifeasible takes plant genetic transformation as its characteristic advantage, and at the same time specializes in various molecular biology experimental techniques, and has advantages in a variety of plant transgenic technologies. We can provide professional, convenient, and comprehensive technical support and services to research institutions and seed enterprises. We cooperate at various levels, including gene cloning, gene editing, vector construction, plant transformation, high-throughput screening, and detection and analysis. If you are interested in us, please feel free to contact us.