Rice anther In Vitro Culture

Rice anther culture is the process of using aseptic operation techniques to inoculate rice anthers that have developed to a certain period onto an artificial medium, inducing dedifferentiation to form healing tissues by altering the developmental program of pollen grains and then allowing the healing tissues to re-differentiate to form haploid plants. Haploid plants undergo colchicine or natural doubling to become complete diploid plants. Combining anther culture with other breeding techniques can rapidly purify breeding materials, effectively shorten the breeding cycle, improve selection efficiency, and accelerate the breeding process.

Conventional rice breeding methods need to be separated for many generations to form stable varieties. At the same time, anther culture technology has the advantages of shortening the breeding period and rapidly stabilizing breeding materials. The use of anther culture technology as a breeding aid is highly effective in the creation of original materials, selection of new varieties, and purification of sterile lines. In response to this situation, Lifeasible has developed rice anther culture technology services, and the in vitro culture of rice anthers that we provide has played an important role in helping our customers promote the progress of rice research.

What do we offer?

We used rice anthers as materials to obtain independent healing tissues quickly and with high quality for further differentiation and regeneration into plants. When rice is in the pregnant spike stage and the leaf cushion spacing is 5-15cm (this period is the mononuclear leaning stage of pollen differentiation), cut the spike, peel off the leaf sheaths, cut the young spike into a length of 5cm, peel off the anthers with pointed forceps, inoculate the anthers into the medium, and when the healing tissues are grown out, transfer them to the differentiation medium, and differentiate the green seedling.

Why choose us?

• We have a professional technical team that has been engaged in plant tissue culture for many years, which can guarantee the experiment's success rate.
• We have an efficient experimental process. Optimized anther culture system, ready to carry out genetic transformation projects.
• We have perfect service support. Provide professional technical advice and guidance in the early stage, communicate with each other at any time during the experiment to keep abreast of the progress, and provide the agreed results according to the contract after the completion of the project.

Our experimental deliverables

• Each spike provides 4-10 seedlings, which is determined by the state of the material.
• Experimental data and pictures.
• Experimental report of the project.

Our service workflow

Lifeasible has a professional and technical team engaged in plant genetic transformation for many years. Our plant genetic transformation platform has efficient experimental processes, professional technical support before and after the sale, and perfect molecular biology experimental equipment. Our experimental staff can customize one-on-one solutions according to our customers' needs, and provide various plant transformation services to meet the needs of our customers. If you are interested in us, please feel free to contact us.