Sesbania cannabina Transformation

Sesbania cannabina is an annual with green or maroon stems, linear-oblong leaflets, asymmetrical on both sides, white sericeous hairs on flowering branches, no prickles on the leaf and inflorescence axes, elongated terete pods, and glossy, black-brown, short cylindrical seeds. Sesbania cannabina is rich in nutrients because it is an excellent summer green manure crop grown in fields. Sesbania cannabina is salt-tolerant, which makes it a pioneer crop for improving saline soil. Especially in low-lying and flood-prone saline areas, planting Sesbania cannabina can significantly reduce the salinity of the soil in the plow layer. In addition, Sesbania cannabina has good feeding, medicinal, and economic value. However, there is a problem of low yield in the production of Sesbania cannabina, and the low yield seriously limits the development of Sesbania cannabina cultivation industry.

Lifeasible has the characteristic advantage of a plant genetic transformation system. We also specialize in various molecular biology experimental techniques. We have the leading technological advantage in Sesbania cannabina genetic transformation and genetic engineering-related technologies, with a high positive rate of regenerated plants. The Sesbania cannabina genetic transformation methods we provide can provide effective solutions to improve Sesbania cannabina yield and promote the development of Sesbania cannabina research and application.

What do we offer?

Agrobacterium transformation method has become a common and effective tool for genetic transformation of Sesbania cannabina, providing an important means for plant gene editing and improvement. We improved and optimized the Agrobacterium transformation method according to the needs of Sesbania cannabina genetic transformation and developed a set of more efficient transformation protocols, which showed good application results in simplifying the experimental operation, improving the efficiency of plant transformation and increasing the applicability of plant transformation. We have the following main steps in the genetic transformation of Sesbania cannabina using Agrobacterium transformation method.

• Preparation of sterile seedlings. Sterilized Sesbania cannabina seeds were inoculated onto solid medium and cultured to obtain sterile seedlings.
• Pre-matching culture of exoskeleton. The cultured sterile seedlings were cut into explant parts, and the explants were inoculated onto a pre-culture medium for pre-culture.
• Infiltration and co-culture. The cultured exosomes were immersed in a suspension containing Agrobacterium carrying the target gene to allow Agrobacterium to infect the plant cells. Agrobacterium transfers the target gene into the plant cells through the T-DNA region of its Ti plasmid. At the end of the infestation the exosomes were drained of water and placed in co-culture medium to obtain co-cultured exosomes.
• Recovery culture. The co-cultured exosomes were restored to culture under light.
• Screening culture. Transfer the recovered tissues to the screening medium and obtain resistant healing tissues after screening culture.
• Differentiation culture. Sesbania cannabina seedlings were obtained by transferring the healing tissues to a differentiation medium.
• Rooting culture. Sesbania cannabina seedlings were transferred to rooting medium to obtain transgenic plants.

What do customers need to provide?

We use the Agrobacterium-mediated method to realize the genetic transformation technology service of Sesbania cannabina. Customers need to provide target genes, plasmids, Agrobacterium, etc., and we will complete the whole experimental process for you, from vector construction to obtaining transgenic plants and receiving seeds. In addition, you can also choose to let us help you with gene cloning and expression, silencing, knockdown, and other kinds of vector constructs to construct vectors, etc., saving you more time.

Why choose us?

• Professional team. We have many years of research experience in Sesbania cannabina genetic transformation, and our professional team can design and execute efficient genetic transformation programs for different needs.
• Customized service. We provide customized Sesbania cannabina genetic transformation solutions according to customer's project needs, to maximize the satisfaction of customer's target plant transformation needs.
• Quality assurance. We strictly control every step to ensure the quality and stability of Sesbania cannabina genetic transformation process, which greatly ensures the success rate of transformation and helps customers save time and cost.

Our service workflow

Lifeasible is an industry leader in plant genetic transformation. Customers can make individual requests for project service options and delivery standards, and we will provide customized solutions to ensure that their experimental needs are fully met possible. If you are interested, please feel free to contact us.