Yeast Display Technology Services

The yeast cell surface display system has a well-established protein post-translational modification and secretion mechanism. Lifeasible introduces exogenous target protein gene sequences (exogenous proteins) into yeast cells after fusion with specific vector gene sequences. The expressed target protein is then localized to the yeast cell surface using the yeast intracellular protein translocation mechanism (GPI anchoring).

Figure 1. Cell surface anchoring systems in yeast. (Lozančić, M. et al., 2019)

We provide yeast display systems, including agglutinin and flocculation display expression system, depending on the site of fusion of the exogenous target protein with the yeast cell wall.

Compared to phage and E. coli cell surface display technologies, we offer yeast display expression systems with the advantages of glycosylation, post-translational folding of proteins, and a secretion mechanism similar to that of mammals. One yeast cell is capable of displaying the expression of roughly 10⁴ lectin proteins, allowing us to express complex proteins with biological activity efficiently.

Wide range of applications

• Alcoholic fermentation. We can use the α-agglutinin display system to display multiple cellulose hydrolases on the surface of Saccharomyces cerevisiae simultaneously. Eventually, it can directly convert amorphous cellulose into ethanol.
• New drug screening. We can display gene products of unknown functional purpose or protein products related to specific pathological processes on the yeast surface. Screening of cDNA expression libraries or mutant libraries will reveal substances interacting with these genes or pathological process-related products of unknown function. It to better assist you in studying unknown gene functions, screening the study of unknown gene functions, or lead compounds for new drugs to treat disease.
• Biosorption. We can use yeast display systems to display heavy metals or certain organic chemicals, directly on the surface. This ultimately assists you in the bioremediation of environmental contaminants.
• Antigen surface displays. We display antigenic proteins on the surface of yeast cells, which allows them to more closely resemble their natural spatial conformation, allowing for serological assays such as ELISA.
• Other aspects. Our yeast display technology services can also be applied to the mutual recognition of protein molecules, directed evolution, and enzyme immobilization.

Lifeasible uses yeast display technology to immobilize and display heterologous eukaryotic proteins on the surface of yeast cells. We meet your research needs in protein library screening, directed protein evolution, high-affinity antibody sorting, antigen/antibody library construction, antibody affinity maturation, vaccine production, immuno-biotechnology, and biosensors. Please contact our staff for your customized solution.