Introductions

Agricultural phages can infest soil with pathogenic bacteria, thus enabling the control of pathogens to ensure crop health. To better understand phages and to determine whether phages contain part of a putative virulence factor, whole genome sequencing is used, requiring high-quality phage DNA. Traditional methods of phage DNA extraction are cumbersome and time-consuming and require specialized equipment such as ultracentrifuges.

Lifeasible has extensive analytical experience in the plant field, and the development of agrophages is beneficial for conducting plant disease control. We provide integrated services, including isolation, identification, purification, culture, and DNA extraction services for agrophages, which help our customers to develop valuable agrophages better.

Services content

The following is the specific procedure for agricultural phage DNA extraction:

• Separate and purify the phage suspended in ribonuclease solution and incubate at 37 °C in a shaker for a certain length.
• Transfer the liquid to a centrifuge tube, add the SDS mixture and leave it in a hot water bath for a certain period.
• Immediately after removal, add potassium acetate and ice bath.
• Centrifuge with a micro high-speed centrifuge, transfer the supernatant to a new tube, mix with an equal volume of phenol/chloroform, and shake manually.
• Centrifuge with a micro high-speed centrifuge, separate the two phases, transfer the supernatant to another new tube and add isopropanol.
• Centrifuge with a micro high-speed centrifuge to precipitate DNA, add a buffer to dissolve DNA, and store at -20 °C.

Things to note for customers

• When the phage and host bacteria used for lysis are obtained in a fresh culture, lysis is good, and lysis phage harvest is enormous.
• When liquid culture lysis, if lysis has not occurred by the time of incubation, the incubation temperature can be appropriately increased, or the shaking speed can be increased.
• When phenol/chloroform extraction, pay attention to the contamination of impurities such as PEG and protein, which can affect restriction endonuclease cleavage.

Service process

Feedback to the customer

Lifeasible can provides professional agricultural phage DNA extraction services for our customers. In addition, we also provide all data reports of the project, including experimental protocols, experimental procedures, and experimental conditions. We can also provide solutions related to DNA provision if you need.

Advantages

• We have unparalleled expertise in agricultural phage DNA extraction and have the expertise to help our customers design superior extraction delivery solutions to meet their needs.
• We have a team of experienced, dedicated, and enterprising professional staff.
• We provide integrated and comprehensive services, including the isolation, purification, identification, and genomic analysis of agricultural phages.
• We have a strict quality control management system, standardized operation procedures, etc.