Separation of alkaloids is a common laboratory technique used to extract and purify these compounds from complex mixtures. Lifeasible offers alkaloid separation services, including consulting and protocol design, sample preparation, sample extraction, alkaloid separation, and analytical characterization services.
Column Chromatography - Alkaloids can be separated and purified in column chromatography by selective adsorption and elution steps.
Thin-Layer Chromatography (TLC) - An alkaloid sample is separated by sublimation, diffusion, or adsorption after being applied to a thin-layer plate and then visualized by detection methods such as color developers or UV light.
High-Performance Liquid Chromatography (HPLC) - Depending on the choice of column packing and solvent system, high-efficiency separation and purification of alkaloids can be achieved.
Gas Chromatography (GC) - An alkaloid sample is evaporated and separated on a gas chromatography column.
Lifeasible consults with customers in advance to understand their research objectives and needs. Based on the customer's requirements, we will design a suitable separation program and provide technical advice and experimental procedures.
Customers are required to provide samples of raw materials, such as plant tissues, animal tissues, or other samples containing alkaloids. We can also assist customers in sample collection, processing, and pulverization if required.
The extracted solution will be passed through to separate the target alkaloids and remove impurities.
The extract is filtered to remove solid residues and plant impurities. Separation can be done using filter paper, membranes, or other suitable filters. The filtered liquid usually contains the target alkaloid and the extraction solvent.
The filtered extract is concentrated to obtain a higher concentration of the alkaloid. Concentration methods may use rotary evaporators, freeze drying, or spray drying. By removing the extraction solvent, the concentration of the alkaloids will be increased.
The concentrated alkaloid extract will be further separated depending on the separation technique used (e.g., column chromatography, thin-layer chromatography, high-performance liquid chromatography, etc.). This may involve the use of different columns, packing materials, and solvent systems to achieve an efficient separation of the target alkaloids.
The specific operating conditions and methods for each step may vary depending on the purpose of the experiment, the characteristics of the samples, and the separation techniques employed. Therefore, in practice, they may need to be optimized and adjusted case-by-case to obtain the best alkaloid separation and purification results.
With state-of-the-art technology and a team of talented scientists and experts, Lifeasible is committed to providing the best alkaloid separation services at competitive prices to customers worldwide. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following: