Analysis of Pine Wilt Diseases

Pine wilt disease (PWD) is a major worldwide quarantine forest disease caused by the pine wood nematode Bursaphelenchus xylophilus. North America is the origin of pine wilt disease, while Japan, China, and South Korea in Asia are the most affected areas. Pine wilt disease develops rapidly after infecting pine trees in Asia, which is harmful and difficult to control, resulting in serious economic losses. Pine wood nematode disease can infect 51 pine species and 16 non-pine species under natural conditions. The pathogenic pine wood nematodes cause pine to wilt quickly, and the pathogenic mechanism is complex.

Lifeasible makes full use of our specialized libraries, selection strategies, and maturation techniques to optimize the stability and performance of plant science. With advanced technology and experienced staff, we provide analysis of pine wilt diseases to global clients to support their research.

Pathogen Analysis of PWD

• Several decades after the epidemic of pine wilt disease in Japan. In 1971, a nematode was isolated from dead pine trees and was identified as the cause of pine wilt disease by artificial inoculation.
• In the diagnosis of pine wilt disease, it is essential to identify pine wood nematode accurately. Lifeasible provides pathogen analysis through artificial inoculation experiments and egg culture technology. There are many nematodes parasitized in pine, among which the nematodes of Bursaphelenchusare the most numerous.
• In addition, we help analyze the presence of associated bacteria in and on pine wood nematodes, which indirectly influence the pathogenicity of the nematodes.

Analysis of Cyclic Infection

Fig.1 Life cycle of the pinewood nematode, Bursaphelenchus xylophilus, and pine wilt disease. (Vicente C, et al., 2012)

• Natural transmission of pine wood nematode disease depends on the transmission of the vector insect between the diseased and healthy plants. The cycle of pine wood nematode infection varies in different countries and regions, but the overall cycle is similar.
• Most pine trees infected with pine wood nematode disease die in the same year of infection. We provide an analysis of the vectors of disease transmission, disease transmission efficiency, and harmfulness, as well as an analysis of the reproductive cycle, reproductive stage, and infection symptoms of insects in pine wood.

Analysis of Pathogenic Mechanism

• In recent years, studies on the pathogenesis of pine wood nematodes have obtained many experimental results in many aspects, especially in the molecular mechanism of the pathogenesis, which greatly enriched the possible comprehensive interpretation of the pathogenesis.
• We provide an analysis of the mechanisms by which pine wilt nematodes weaken the mechanical strength of cell walls and protect themselves from defense-related compounds secreted by the host, such as the endogenous β-1,3-glucanase gene, glutathione S transferase, and others.
• As a class of secreted proteins that play an important role in pathogen infection, we help our clients to analyze several pine nematode candidate effectors, including cytochrome P450 gene, lysozyme protein gene, VAP protein gene, glutathione transferase gene,etc.

Lifeasible offers a reliable analysis of pine wilt diseases to meet your research demands. With years of experience in plant science, our advanced platforms can help our clients solve various difficulties and conduct research. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.