Bioinformatics Analysis of Higher Plant ANS Gene

Anthocyanins are of great importance to plants and humans. It can give plants colors, attract various insects to pollinate, disperse seeds, and help plants resist adversity. It also plays a vital role in anti-inflammatory, antibacterial, anti-oxidation, anti-aging, and other aspects. Anthocyanidin synthase (ANS) is a key enzyme in anthocyanin synthesis. With the publication of a large number of plants' ANS genes, more and more botanists began to analyze the biological characteristics of ANS.

Lifeasible is a world-renowned provider of plant bio-services in all aspects of plant research. Based on our professional bioinformatics staff and extensive technical expertise, we offer our clients a customizable bioinformatics analysis service for ANS genes in higher plants.

What We Offer

To identify and analyze the ANS genes of the target plants, we will obtain the ANS gene sequences and ANS amino acid sequences of dozens of higher plants, such as papaya, money orange, sweet orange, apple, peach, and grape, which have been published on NCBI by BLAST method. The phylogenetic analysis, physicochemical property analysis, and structural analysis of the target ANS genes are carried out using the ANS genes of Arabidopsis thaliana as a reference. The target ANS amino acid sequences' subcellular localization, hydrophilicity/hydrophobicity, protein secondary and tertiary structures, and phylogeny are also analyzed.

• Analysis of the Basic Properties of ANS

1. Physicochemical properties of ANS cDNA and its coding amino acid sequence.
2. Nucleotide sequence alignment analysis of ANS. The nucleotide sequences of these plant ANS are compared for homology using Bio edit software.
3. Hydrophobic/hydrophilic characterization of ANS. The hydrophobicity/hydrophilicity of the ANS of these plants is analyzed online using ProtScale.
4. Predict structural domains of ANS across membranes.
5. Structural domain characterization of ANS.
6. Signal peptide and guide peptide characterization of ANS. Analysis of the location and sequence of the signal peptide present in the target ANS amino acid sequence signal peptide using SignalP 3.0 Server.
7. ANS subcellular localization analysis.

• ANS Protein Secondary Structure Characterization

Use SOPMA to predict the most secondary structure elements of a target ANS.

• Construction of ANS Protein Phylogenetic Trees

The ANS nucleotide sequences of these plants are compared in multiple sequences using MEGA 12.0 software, and a phylogenetic tree was constructed using default parameters.

Applications

• Improving the resistance of plants to stress.
• Improving the efficacy of medicinal plants.
• The exploitation of anthocyanins.
• Exploring the molecular regulation of anthocyanin synthesis.

Why Choose Lifeasible

Based on the high homology of ANS genes in different plants, we selected dozens of species to compare the characteristics of ANS genes in the target plants with those in other plants, which is more relevant and informative. Furthermore, we have fully and systematically analyzed the basic properties, protein secondary structure, tertiary structure, and phylogeny of ANS genes in the target plants to provide theoretical support for the mechanism of the role of ANS in the anthocyanin synthesis pathway.

How to Place an Order

Lifeasible always maintains a customer-focused service philosophy and does its best to provide cost-effective bioinformatics analysis of ANS genes in higher plants. For more information, please contact us directly.