Biomolecular Sensors Based on Nanopore Sequencing

Rapid detection of plant pathogens is essential for disease control and phytosanitary work. Plant pathogen detection requires discovering and identifying potential biohazardous factors (including known or unknown species of bacteria, fungi, and viruses) from plant samples. Due to the complex interactions between plants and environmental microorganisms, how to rapidly screen and accurately identify the target pathogenic microorganisms is a complex problem in front of the technicians. Nanopore sequencing technology can analyze complex nucleotide groups efficiently, rapidly, and comprehensively compared to other pathogenic microbial detection methods. Lifeasible's nanopore technology expands the idea of detecting plant pathogenic microorganisms that can't be isolated and cultured and also provides a means to accurately identify viruses, which can satisfy your needs for detecting bacteria, viruses, and multiple viruses in complex infected plant tissues.

Figure 1. Principle of nanopore sequencing.

We extract DNA or RNA from the sample to be tested and then construct a simple library for online testing to obtain information on all pathogenic microorganisms in the plant sample. This simplifies the research process, shortens the research period, and detects more genes and unknown sequences than other methods.

Application examples

• We used metagenomics sequencing technology to detect cowpea bright yellow mosaic virus (CoBYMV) in Vigna unguiculata.
• We used nanopore sequencing technology to sequence cDNA from infected wheat tissues and detected the presence of wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and barley yellow dwarf virus-PAV (BYDVPAV) complex infection.
• We used nanopore sequencing to sequence the metagenomics of Solanum lycopersicum and other crop pathogens (including bacteria, viruses, fungi, and phytoplasmas), which provides accurate identification of all inoculated pathogens within a 1-2 h sequencing time.
• We sequenced the metatranscriptome of Dioscorea alata using nanopore sequencing combined with a cDNA-PCR library construction strategy to identify its RNA virome. Using this method, it was also possible to detect the complex infestation of Dioscorea alata by Dioscorea bacilliform virus (DBV), yam mild mosaic virus (YMMV), and yam chlorotic necrosis virus (YCNV).

Rapid detection of plant pathogens is a prerequisite for plant disease control. Nanopore sequencing is a real-time sequencing technology that converts nucleic acid base signals into electrical signals, with the advantages of ultra-long sequencing read lengths and real-time sequencing, allowing rapid determination of the full-length sequences of DNA and RNA. Lifeasible offers nanopore sequencing-based rapid plant pathogen detection with high accuracy, multiple targets, and real-time analysis of test results. Please feel free to contact us with any information about the plant pathogens you would like to test for so that we can quickly customize a test for you.