Brassica oleracea, known as kale, is a common leafy vegetable crop. Kale is relatively high in nutrients and contains a large amount of vitamin C, E, and B. Currently, kale is widely grown worldwide, and great progress has been made in conventional breeding. However, the years of conventional breeding are very long. Therefore, plant genetic engineering technology would be a better choice to make new breakthroughs in the selection and breeding of insect and disease resistant kale varieties. Transgenic system can break the bottleneck of kale breeding and finally achieve a big speed-up in the kale breeding process.
Lifeasible has a proven kale genetic transformation system that allows us to provide large-scale, standardized kale genetic transformation services. As your best partner in plant genetic transformation, we can help you efficiently obtain transgenic kale plants that meet your requirements quickly. Our versatile kale gene transformation technology platform revolves around the Agrobacterium transformation method, using kale leaves as the recipient material. Immediately after the Agrobacterium-mediated genetic transformation of kale, we perform the corresponding resistance screening. Subsequently, independent resistant healing tissues are obtained, which are eventually further regenerated into transgenic kale. Here our various kale genetic modification services are listed below.
Customers can provide constructed kale transformation vectors directly, and Lifeasible also offers vector construction services.
| Concentration | Volume | Other requirements |
| 80-100 ng/μL | ≥10 μL | no degradation & no contamination |
| Bacterial broth | Bacterial plate |
| Bacterial broth up to 1-year-old, preserved in glycerol | Bacterial plate activated within one week |
*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.
*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.
*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.
Experts are available throughout the service process of kale transformation to provide you with project consultation, experiment design, experiment customization, experiment operation and progress tracking. You can contact us anytime for real-time progress, and we will keep you informed regularly, especially at critical points of the experiment. The standardized experimental procedure is shown in the above diagram, you just need to submit the samples as required, and then you will get the positive seedlings and the experimental report within the agreed time.
Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in brassica oleracea transformation. We can provide customized solutions to help you study a wide range of kale varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
