Food Safety and Nutrient Detection

ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy

Reduced Glutathione Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at 450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. Our kit is the most specific and sensitive quantitative assay in the market because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples’ GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.

Sodium Assay Kit offers a simple, two-step colorimetric assay that is based on the requirement of sodium ion as a cofactor for the enzymatic activity of β-Galactosidase (βG). Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay. The kit can detect Sodium concentration as low as 25 µM in a variety of samples.

Taurine Assay Kit enables the measurement of Taurine level in both biological fluids as well as food products, such as energy drinks. The kit utilizes the ability of an enzyme to convert Taurine into aminoacetaldehyde and sulfite. The produced sulfite is measured using a probe which can be detected using a microplate reader (OD 415 nm). This assay kit is simple, high-throughput compatible and can detect as low as 5 nmols of Taurine.

This TAC Assay Kit can measure either the combination of both small molecule antioxidants and proteins or small molecules alone in the presence of our proprietary Protein Mask. Cu²ᶧ ion is converted to Cu+ by both small molecule and protein. The Protein Mask prevents Cu²ᶧ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.

In Total Carbohydrate Assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural, which react with the Developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm. The Total Carbohydrate Assay can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.

Total Sulfite Assay kit is a simple and sensitive assay to detect small concentrations of sulfites in a variety of food and beverage samples. This assay is based on the oxidation of sulfite to sulfate producing a stable signal at 570 nm, which is directly proportional to the amount of sulfite in the sample. This assay is very sensitive and can detect as low as 20 µM of sulfite in a variety of samples.

The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Zinc Fluorometric Assay kit provides an easy and quantitative method to measure the metal ion Zinc in various biological samples. The assay is based on the ability of our proprietary probe that binds with high specificity to Zinc and becomes fluorescent. The fluorescent intensity is directly proportional to the amount of zinc, which can be quantified by measurement at Ex/Em= 435/535 nm in a microplate reader. This assay kit provides a just add-and-read, non-radioactive, and high-throughput adaptable for Zinc detection. The assay is rapid (less than 10 min) and sensitive (Limit of Detection: <0.1 µM)

Zinc Assay Kit is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm. The assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).