Food Safety and Nutrient Detection

β-lactamase Rapid Detection Kit is designed for the detection of β-lactamase in milk.

Tryptophan Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects tryptophan concentration in biological fluids, including free and bound tryptophan in serum. The principle is based on the non-enzymatic reaction to generate a fluorophore (Ex/Em = 370/440 nm) using tryptophan as a building block. The reaction is specific and other amino acids do not interfere with the assay. The assay can detect as little as 2.5 µM of TRP in a variety of biological samples.

β-lactam antibiotics Rapid Detection Kit is designed for the detection of β-lactam antibiotics in milk.

Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 µM.

α-amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by α-amylase, the smaller fragments produced can be acted upon by α-glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect α-amylase content as low as 0.2 mU.

Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.

BCAA Assay Kit provides a simple convenient means of measuring the BCAA’s in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA’s are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

Cysteine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of cysteine in a variety of biological fluids. The principle of the assay is based on the cleavage of thiol group of reduced cysteine producing a fluorophore (Ex/Em = 365/450 nm) with a stable signal, which is directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. The assay can detect as little as 10 µM of Cysteine in a variety of samples.

DL-Serine Assay Kit allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 539/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 μM Serine.