Cell-Free Protein Expression

Cell-free protein expression (CFPS) is a technique that mimics the transcription and translation processes in cells under in vitro conditions to synthesize proteins. Instead of relying on living cells, this technology utilizes biomolecular translation mechanisms extracted from different cell types, such as wheat germ and plant cells, to express target proteins in an in vitro environment. Cell-free protein expression meets the growing need for a more direct, rapid, and efficient way to produce proteins, overcoming many barriers to traditional cellular expression.

Lifeasible has extensive experience in providing services for the study of plant protein expression systems, and we are constantly optimizing and updating our plant protein expression technology services to work with researchers to better and faster advance cell-free protein expression in plants.

Features of Cell-Free Protein Expression

• Toxic protein expression. Toxicity may arise from misfolded proteins, aggregation, or proteins interacting with critical cellular pathways. Cell-free systems provide a controlled environment in which protein expression can be fine-tuned to avoid over-accumulation or destabilizing effects of highly toxic proteins.
• High-throughput protein expression. In cell-free expression systems, multiple proteins can be expressed in parallel quickly without the need for cloning, transformation, or cell culture. This greatly accelerates discovering proteins with desired properties or that are difficult to express in living cells.
• Protein expression with appropriate post-translational modifications (PTM). Post-translational modifications (PTM) are critical to the functional diversity of proteins, affecting their activity, stability, and interactions. The incorporation of specific PTM pathways in cell-free systems is promising and can be used to produce proteins with complex modifications by optimizing the expression environment with the necessary cofactors and enzymes.

Our Service for Cell-Free Protein Expression

We offer a cell-free expression system that produces proteins in vitro through a translational mechanism derived from cells, which is now becoming a well-established technology for synthesizing proteins in cell lysates. This technology service has demonstrated multiple advantages in protein production, including the fact that the cell-free expression system is ideal for high-throughput screening of mutagenesis, and the fact that the cell-free expression system works independently and is not affected by the cellular environment. The entire mechanism of cell-free expression systems establishes a better environment for making membrane proteins and proteins that are easily degraded by intracellular proteases. During the process, we can achieve simple manipulation of the reaction steps by adding or removing certain factors such as cofactors, energy sources, detergents, etc.

• Tobacco Cell-Free Protein Expression

Tobacco cell-free protein expression utilizes extracts from tobacco plant cells to efficiently produce recombinant proteins outside living cells, providing a scalable and versatile platform for high-yield protein synthesis.

• Wheat Germ Extract Cell-Free Expression

Wheat germ lysate is another popular eukaryotic expression system, yield higher than any other eukaryotic system. Also, it is ideal for the expression of large-size proteins.

Application of Cell-Free Protein Expression

The cell-free protein expression service we provide has a wide range of applications in various fields such as biopharmaceuticals, crop development, metabolic pathways, and basic research. It can provide high-throughput protein expression of different types of proteins, including reporter genes, antigens, antibodies, enzyme molecules, membrane proteins, and so on. In addition, cell-free protein expression technology can also be used to synthesize important biomedical proteins providing an efficient and controlled protein synthesis platform for drug discovery and biomedical research.

Highlights of Our Cell-Free Protein Expression

• Customized service. We provide customized protein production and synthesis services to meet our clients' research needs.
• Rapid protein synthesis. With this service, we can help researchers eliminate the time spent on cell growth and protein expression cycles, making protein production more rapid.
• Flexibility. We have higher flexibility in experimental processing, allowing us to carry out reactions at different temperatures, pH, and other conditions and to adjust the concentration of added substrates and cofactors to optimize protein expression.
• High-quality protein production and purification process. We have high-quality plant-derived protein production and purification processes to provide customized protein expression services.

Fig.2 Our service advantages. (Lifeasible)

Lifeasible is an industry leader in cell-free protein expression, and we can provide you with the ultimate solution for cell-free protein production, as well as customize your solution to meet your specific requirements, such as labeling options, purity, and other requirements. If you are interested, please feel free to contact us.