Construction of Engineered Strains of Riboflavin E. coli

Riboflavin is a member of the B vitamin group, which is an essential nutrient for mammals. In the last decade or so, the chemical synthesis of riboflavin has been completely replaced by microbial synthesis. In recent years, the production cost of riboflavin has continued to rise. Therefore, further improvement of riboflavin production strains and reduction of fermentation production costs have become urgent.

Lifeasible used synthetic biology techniques to construct an engineered strain of E. coli with high riboflavin production, to structurally optimize and chemically synthesize multiple genes of the ab initio biosynthetic pathway of riboflavin in Bacillus subtilis, and to reconstruct the riboflavin biosynthesis system in E. coli. Ultimately, in vitro targeted molecular evolution of the relevant RNA polymerase, riboflavin ab initio biosynthesis system, and global transcriptional regulators will be performed by DNA rearrangement technology to obtain an engineered strain of E. coli with excessive riboflavin production.

Features of constructing an engineered colony of riboflavin E. coli

Compared with the chemical semi-synthetic method, riboflavin production using synthetic biology technology has the following characteristics.

• Cheap raw materials.
• No pollution to the environment.
• Simple production process.
• Short production cycle.
• High product purity.

DNA shuffling based in vitro directed molecular evolution technique

• DNA shuffling is a technique used for in vitro recombination of individual genes or homologous gene libraries. These genes are divided into random-sized fragments, and the polymerase chain reaction (PCR) recombines full-length genes from the fragments by self-initiation, resulting in recombination due to PCR template conversion. After screening these PCR products and sequencing the products of interest, improved clones are reshuffled to recombine useful mutations in an additive or synergistic manner, effectively mimicking the natural process of sexual recombination. DNA recombination is a powerful tool for the rapid targeted evolution of new genes, manipulators, and entire genomes.
• Experimental procedure for DNA recombination technology

Advantages of our services

• High accuracy rate of experiments. Ensuring the greatest degree of data fairness and reliability.
• Fast service cycle. The shortest time for customers to deliver satisfactory experimental results.
• Customized service. We provide flexible and customized experimental solutions according to customer project needs.

Lifeasible is dedicated to the agricultural synthetic biology field. Our goal is to help researchers worldwide provide advanced synthetic biology technologies and effective solutions to produce a large number of high-value biological products to promote sustainable agricultural development. Our skilled scientists provide technical support and theoretical strategies to promote the development of synthetic biology through the modification of engineered strains of E. coli by synthetic biology and genetic engineering-related techniques. If you are interested in us, please feel free to contact us.