Cotton Molecular Characterization Analysis

Cotton, including Upland cotton (Gossypium hirsutum) and Sea Island cotton (Gossypium barbadense), is a commercial crop of world importance. It is the world's major textile fiber crop and a source of minor products such as oil, live animal feed (cottonseed cake), and cellulose. Although classical breeding has contributed greatly to quality improvements and increased yields in the twentieth century, textiles require further improvements in fiber strength, length, chemical compatibility of dye binding, water absorption, thermal properties, and resistance to wrinkles and shrinkage sex. The potential to improve these traits through classical breeding is limited due to requirements for species compatibility and availability of traits. Genetic engineering will provide new methods to overcome the shortcomings of classical breeding by introducing target genes from different sources into cotton. However, there are fewer studies on the integration structure of exogenous genes in transgenic cotton, the molecular characterization of exogenous insertion fragments, etc. Figuring out the integration structure of exogenous genes in transgenic cotton will help to establish a detection method for transgenic cotton, which is of great significance for transgenic biosafety.

Fig. 1. Histochemical localization of GUS gene expression in transgenic cotton plants containing the GhTUB1/GUS fusion gene. (Li et al., 2002)

Our Solutions

We provide advanced plant genetic engineering services to improve cotton genetic traits by applying gene gun, pollen pipeline, Agrobacterium-mediated methods, etc. We can help you breed high-quality insect-resistant, herbicide-resistant cotton, etc. With years of experience, Lifeasible provides professional cotton molecular characterization analysis services to evaluate the safety of genetically modified cotton. We can provide you with cotton molecular characteristics information at the chromosome level, including the T-DNA insertion site, copy number, and flanking sequences of foreign genes.

We have a complete genetically modified corn molecular characterization detection system, including Southern hybridization, Polymerase chain reaction (PCR) analysis, PCR-based genome walking, quantitative real-time polymerase chain reaction (qRT-PCR), Southern hybridization, Western blotting, ELISA, high throughput sequencing, etc. Based on your project requirements, we will select the most suitable detection method for analyzing molecular characterization of genetically modified cotton. We can analyze the following genetically modified cotton, including but not limited to:

Work Process of Genetically Modified Cotton Molecular Characterization Analysis

1. Collection of genetically modified cotton material.
2. RNA isolation and construction of cotton cDNA library.
3. Isolation and Northern blot analysis of cDNA clones of foreign genes.
4. RT-PCR analysis.
5. DNA isolation and Southern blot analysis.
6. Construction and screening of cotton genome library.
7. Genome Walker library construction and Genome Walker PCR.
8. DNA sequence analysis.

Lifeasible aims to obtain the integrated structure and molecular characteristics of exogenous genes in genetically modified cotton and to establish the specific detection method for transformation events and the screening method for pure congeners. Please contact us for the best analysis solutions for genetically modified cotton molecular characterization.