Culture and Establishment of Fish Cell Lines

The relative abundance and broad biochemical and genetic characterization of mammalian in vitro cellular systems contrasts with the information available for other vertebrate organisms, particularly fish (the most diverse and oldest vertebrate class). Fish share many essential characteristics with mammals (e.g., similar organ systems, developmental organization, and physiological/biochemical mechanisms) and exhibit a variety of technological advantages, including adaptation to a wider range of temperatures, greater tolerance to hypoxia, and greater ease of maintaining cell cultures for extended periods. Increasingly, fish cell lines are being established from different aquaculture species and used for in vitro studies related to aquaculture and other interdisciplinary fields. Fish has become a promising vertebrate model for most biological studies and a suitable alternative to mammalian systems.

Fig. 1. Implications of fish cell line in aquaculture. (Goswami et al., 2022)

Our Solutions

Our experts are excited in establishing and characterizing new fish cell lines representing different fish species and tissue types. Lifeasible offers customized solutions for the culture and establishment of fish cell lines. In addition, we fully characterize and validate these cell lines to ensure reproducibility without compromising scientific research.

• Culture and Establishment of Fish Cell Lines

We use different fish tissue samples (including gills, caudal fins, eyes, liver, kidneys, intestines, brain, vertebrae, and oro-nasal) to develop and establish fish cell lines. Both primary (cells isolated directly from host tissue) and established cell cultures (immortalized) are available for research. Below is a list of our available fish cell lines, including fish species and source tissues. We also offer cell cultures from marine fish.

List of cell lines developed from warm-water and cold-water fish species:

List of cell lines developed from marine fish:

• Characterization of Fish Cell Lines

Research and development using cell lines requires a detailed knowledge of the purity and originality of the cell line. We offer reliable solutions for fish cell line characterization based on standard protocols for characterization and identification. Some of the standard methods include cytochrome C oxidase subunit 1 (CO1) barcoding, short tandem repeat (STR) analysis, karyotyping, single nucleotide polymorphism (SNP) analysis, use of species-specific primers, whole genome sequencing (WGS), CLASTR, DNA-based authentication, etc.

Advantages of Fish Cell Cultures

• Avoid social and ethical issues of using animals in research and some regulatory issues.
• Easy to administer drugs.
• Reproducible.
• Rapid test results.
• Economic viability.
• Easy to generate, maintain, genetically manipulate, quantify, characterize, and cryopreserve.
• Can be cultured at a wide range of temperatures and osmolalities.
• Easily adapted to bicarbonate buffered media.
• Fish cell culture methods are like those used for terrestrial vertebrates.
• Fish cells have a lower metabolic rate and can be maintained for longer periods.

Applications of Fish Cell Cultures

• Immunology
• Toxicology
• Genetic engineering
• Genetic regulation
• Gene expression research
• Endocrinology
• Biomedical research
• Disease control
• Biotechnology
• Biomedical research
• Radiation biology
• Fish health management
• Cellular aquaculture

Lifeasible explores fish cell lines to produce a variety of new products. We use standard protocols to maintain consistent quality and validate cell lines throughout their in vitro life cycle. Contact us today to learn more about our solutions.

Reference

1. Goswami, M., et al. "Role and relevance of fish cell lines in advanced in vitro research." Molecular Biology Reports. 2022: 1-19.