Flow cytometry has become a useful, rapid and novel method in recent years. It can be effective, reproducible to determine the relative nuclear DNA content and ploidy level of a large number of samples (plants and animals) at low cost. Based on the detection of nuclear DNA content, the ploidy level of a specific plant species can be calculated with high reliability.
The ploidy levels were analyzed using sample of known ploidy (the same or closely related species) as the external standard and DAPI as the fluorochrome. The ploidy level of sample was calculated according to the equation: Sample ploidy level = (Sample G peak mean / Standard G peak mean) x standard ploidy level.
Lifeasible has devoted itself to the development of plant analysis technology and has accumulated rich industry experience. Our company's technology for ploidy analysis using Partec CyFlow Ploidy Analyser has been quite mature. We can help customers analyze the ploidy status of samples with high throughput and quickly. The specific experimental procedures are as follows:
The ploidy levels of control group and samples will be showed in the table and flow cytometric histograms down below.
| Sample Number | Ploidy Level |
| Control | 1N |
| Sample | **N |
Figure 1 Flow cytometry graphs of control group and samples
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Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
