GUS staining is a very powerful tool in plant molecular biology, delivers great versatility for various applications (1). As a sensitive and reliable method that is also easy to perform, GUS staining has become the most popular way to evaluate gene expression in plants.
GUS is short for β-Glucuronidase, a gene from Escherichia coli (E.coli) that encodes a sugar consuming enzyme–Glucuronidase. As there is no detectable GUS gene expression in plants, and the bacterial GUS gene does not interfere with plant intrinsic gene expressions, GUS provides an ideal reporter, being orthogonal to native genetic machinery, for gene expression in the plant system. By fusing the promoter of genes of interest to the GUS coding region, the expression patterns of plant genes can be reflected by the activation of GUS.
With years of experience of plant molecular biotechnology, Lifeasible provides high quality services for GUS staining with a wide range of plant materials (suspension cells, protoplasts, callus, plant organs and whole plants). Adapting to differential research purposes from each project, we offer multiple substrates for both GUS histochemical assay and GUS fluorimetric assay (2), with optimized experimental buffers and protocols.
Figure 1. The GUS staining assay of Arabidopsis ACC synthase genes (3).
As a leading plant biotechnology company with robust technical establishments, Lifeasible proudly offers one-stop services that cover every step of plant GUS staining assays, including:
Meanwhile, plant scientists and experts from both Lifeasible and our distinguished collaborators worldwide will work closely with the clients to ensure that sufficient intellectual and technical assistance is given to each project. Together, we guarantee the quality and success of your research work.
References
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following: