Northern blot is a commonly used molecular biology approach for the evaluation of gene expression at transcriptional levels (1). Northern blot is also known as RNA blot, as it reflects gene expressions via detection of the mRNA levels of target genes. A routine Northern blotting procedure starts with total RNA extraction, which is followed by separation of RNAs by electrophoresis. The blotting step refers to the transferring of separated RNAs to a nylon membrane, which is then exposed to specific probes for RNA detection. The probes can be either DNA or RNA with complementary sequences to target RNAs, and are often tagged with radioactive isotopes or chemiluminescent group (Figure 1).
Figure 1. A schematic description of the Northern blot assay (from Wikipedia)
Compared to other protein expression assays, the Northern blot technique have the following advantages (2):
Lifeasible, as a leading plant biotechnology company, has years of experience in plant molecular assays, as well as well-established platforms with advanced experimental equipment and solid technical support. To save your time and effort, we proudly provide you with one-stop Northern blot services that covers the following aspects:
Moreover, to cope with different research goals, and specific status of each project, we offer customized protocols with optimized experimental equipments, buffers and solutions. our team of skilled scientists and experts are always ready to contribute their intelligence and efforts to your research.
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Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
