A Sequence Characterized Amplified Region (SCAR) is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. SCAR are DNA fragments amplified by the PCR using specific 15-30 bp primers, designed from nucleotide sequences established from cloned RAPD fragments linked to a trait of interest. By using longer PCR primers, SCAR does not face the problem of low reproducibility generally encountered with RAPD. Length polymorphisms are detected by gel electrophoresis.
Figure 1. Comparison of DNA-based molecular markers. Kiran U, et al. 2010)
Compared with RAPD markers, the specific primers used for SCAR markers were longer than the random primers of RAPD by about 10 bp, which improved the annealing temperature and the rigor of the reaction, detected a single band, and might be converted into co-dominant markers, and could be used for molecularly assisted selection breeding.
As one of the PCR-based genetic markers, SCAR could be derived from RAPD, ISSR, SCoT, IRAP, and ILP markers. Compared to conventional molecular markers, SCAR has proven to be simpler, more reproducible, and more reliable when used for plant identification at the intra-and/or inter-specific level. Besides, SCAR does not require radioisotopes and detects only a single locus, indicating that SCAR is highly reproducible and locus-specific and can be applied in mapping studies and marker-assisted selection for genetic agroeconomic traits such as bio resistance in various crops.
Lifeasible offers a full range of SCAR technology services based on a proven molecular marker research platform, from plant genomic DNA extraction and purification, specific primer design, to the development of sequence-characterized amplification regions. We also focus on the establishment and development of SCAR markers for different species and provide SCAR marker-based molecular identification services.
Lifeasible is a world-leading phytotechnology company with extensive experience in SCAR technology analysis and a proven, state-of-the-art platform. We provide a full range of SCAR analysis services from experimental design to data analysis. You only need to provide the starting material sample, DNA sample, pre-amplification product, and after the experiment, our customers can get the raw data sheet, DNA electrophoresis test chart, pre-amplification product electrophoresis test chart, sequencing front chart of samples to be sequenced, sequence comparison result and complete result analysis report in the first time.
Lifeasible offers complete, professional SCAR technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact Lifeasible.
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Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
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