Sequence Related Amplified Polymorphism (SRAP) is also known as Sequence Based Amplified Polymorphism (SBAP). This marker amplifies ORFs, which are important components of genes, through a pair of uniquely designed forward and reverse primers, and the genetic diversity of ORFs better reflects the diversity of genetic resources. Since the reverse primer used in SRAP markers contains AATT at the 3' terminal to specifically bind the AT-rich region. Such a unique primer design makes it possible to amplify intron and exon-based SRAP polymorphic markers. At the same time, the primers are longer, 17 or 18 bp, and can be used in combination, which greatly reduces the cost of primer development.
Figure 1. The attributes of different dominant molecular markers used in plant biology, and a relative comparison of their attributes. (Robarts D. W. H, et al. 2014)
Therefore, SRAP can be used in many fields such as genetic diversity analysis, genetic map construction and gene localization, identification of good germplasm and varieties, and prediction of hybrid advantage due to the advantages of easy operation, good reproducibility, efficient co-display, and easy cloning and sequencing.
SRAP markers show relatively more informative bands both at the DNA level and at the cDNA level, thus providing a more comprehensive indication of the degree of polymorphism in the genome. Lifeasible provides professional SRAP technology services to customers worldwide, performing SRAP analysis on 6% denaturing acrylamide gels, which is suitable for amplicons of 100-1000 bp. We have established reproducible and stable SRAP-PCR systems for multiple species and can easily obtain bands and polymorphic information of your interest with our professional direct sequencing platform.
Lifeasible has rich experience in biomarkers, we have more than 200 pairs of SRAP primer combinations, all of them are fluorescent markers, and the solutions we provide are personalized, during the project the customer can synthesize the corresponding primers according to the experimental requirements. To ensure high completion of the project, we usually use about 20 samples before the project starts to do pre-experiments to screen primer combinations with obvious difference bands and clear amplification, and then start formal experiments after the primers are selected. Subsequent sequencing and data analysis are performed by a professional team in strict accordance with the standards to ensure the accuracy of the data.
Lifeasible offers complete, professional SRAP technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact our staff.
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Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
