Simple Sequence Repeats Technology Service

Simple Sequence Repeats (SSR) marker is a molecular marker technique based on specific primer PCR developed in recent years, also known as microsatellite DNA, which widely exists in eukaryotes and some prokaryotes genomes, randomly distributed in nuclear DNA, chloroplast DNA and mitochondrial DNA, composed of 2-6 nucleotides tandem repeat fragments. SSR molecular marker technology has been widely used in the study of plant genetic diversity, genetic linkage map construction, DNA fingerprinting, and variety identification and genome association analysis because of its high polymorphism, good repeatability, relatively simple operation, low cost, and so on.

Figure 1. Schematic workflow of perfect SSR selection, Multiplexed PCR design, high-throughput sequencing, and authentic SSR genotype. (Yang JJ, et al. 2019)

Microsatellite loci are usually amplified by PCR, and the amplification products are analyzed by electrophoresis and detected by segregating alleles according to size. Due to the variation in the number of repeat units of individual microsatellite loci, the variation in the length of the amplification products of individuals gives rise to polymorphism in length, and this polymorphism is called Simple Sequence Repeat Length Polymorphism (SSLP), and each amplified locus represents a pair of alleles of this locus. Because the number of SSR repeats is highly variable, SSR markers can reveal much higher polymorphism than RFLP.

• Technical Characteristics
• Abundant in number, covering the entire genome, revealing high polymorphism.
• Having the characteristic of multiple alleles, providing a high amount of information.
• Inherited in a Mendelian manner with co-dominance, allowing the identification of pure and heterozygous individuals.
• Good experimental reproducibility and reliable results. Each locus is determined by the sequence of designed primers, which facilitates different laboratories to communicate with each other and cooperate in developing specific primers.
• What We Offer

Lifeasible provides SSR assay analysis services based on our advanced sequencing platform, you only need to provide samples, we can provide you with one-stop services from experimental design, experiment implementation to data analysis. We will help you solve any technical and service questions, update project information regularly, and track the progress of your project promptly.

Project	Description
SSR loci search and SSR primer design	Common/fluorescent-labeled primers
Experiment optimization	PCR condition optimization
PCR amplification	Fluorescence or non-fluorescence
Capillary electrophoresis detection	Isolation and detection of monochrome or multicolor fluorescent-labeled DNA fragments by the sequencer
Raw data analysis	Strip interpretation
Population genetics analysis	Association analysis, linkage map construction, etc.

• Service Flow



• Applications
• Genetic crossbreeding
• Mapping chromosomal genetic profiles
• DNA fingerprinting and variety identification
• Germplasm conservation and utilization
• Genomic association analysis
• Why Choose Lifeasible

Lifeasible, as a world-leading phytotechnology company, has rich experience in SSR assay analysis and a mature and advanced platform. We provide a full set of SSR assay analysis services from experimental design to data analysis.

• Wide range of technical services. We use the service to develop markers for genomic unknown species.
• High screening rate. The screening rate is significantly higher than traditional methods.
• High data output and high quality.
• Continuous innovation and progress. We also work on developing new molecular markers.
• Professional and personalized. We have a more professional analysis system and a more personalized analysis service.
• How to Place an Order

Lifeasible offers complete, professional SSR technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact Lifeasible.