Angelica gigas Nakai Transformation

Angelica gigas Nakai is a member of the Umbelliferae family and is easily recognized by its dark purple flowers; the Chinese and Japanese Angelica varieties have white flowers. Angelica gigas Nakai also has many pharmacological properties, such as the treatment of menopausal syndrome, anemia, and abdominal pain, in addition to inhibiting breast cancer and amenorrhea. As a highly valued medicinal plant, Angelica gigas Nakai is known for its therapeutic properties. It produces pyranocoumarins, including decursin and decursinol angelate, which have been found to have significant neuroprotective, anticancer, and anti-androgen receptor signaling activities. The genetic transformation of Angelica gigas has excellent potential to increase the production of these valuable metabolites and enhance their medicinal availability.

Fig. 1. Development of hairy root from a leaf of A. gigas after inoculation with Agrobacterium rhizogenes R1601 containing GUS. (Park N I, et al, 2010)

Solutions

Lifeasible is an industry leader in plant genetic transformation, providing comprehensive genetic transformation services for Angelica gigas Nakai. Our experts are also committed to exploring the Chinese and Japanese Angelica varieties genetic transformation. We offer customized transformation protocols tailored specifically for Angelica gigas, designed to optimize the efficiency and stability of transformation and ensure the reliable production of transgenic plants.

We offer Agrobacterium tumefaciens-mediated transformation services for Angelica gigas Nakai, covering key steps including:

• Exosome Preparation and Co-culture
  Starting with the preparation of exosome tissues (usually leaves or stems), which are then co-cultured with Agrobacterium tumefaciens carrying the desired target gene. Hairy root cultures derived from Agrobacterium tumefaciens-mediated transformation offer several advantages, including genetic and biochemical stability, rapid growth rate, and efficient metabolite production. The bacteria transfer specific genes into the plant cells during the co-culture process.
• Selection and Regeneration
  To ensure successful integration of the target gene, a suitable selection agent, such as kanamycin, is used. Transgenic roots resistant to the selecting agent are then selected and further cultured to promote regeneration and growth.
• Validation of Transgene Integration
  We use various molecular techniques, including PCR analysis, to confirm the transgene integration in the transformed roots. This step ensures the presence and stability of the desired gene in the plant genome.
• Metabolite Analysis
  After successful genetic transformation, we perform a comprehensive metabolite analysis to evaluate the production of valuable compounds, such as decursin and decursinol angelate. Techniques such as HPLC are used to quantify and characterize these metabolites.

Lifeasible provides tailored solutions and comprehensive services for Angelica gigas Nakai genetic transformation. By utilizing cutting-edge technology and customized solutions, we aim to generate Angelica gigas for you with desired traits or metabolites, such as the potential for enhanced pyranocoumarin biosynthesis. If you are interested in our solutions, please contact us for technical consultation and quotation.