Pleurotus tuoliensis Transformation

Pleurotus tuoliensis is named for its resemblance to a Ganoderma lucidum and its pure white color all over. The flesh of Pleurotus tuoliensis is tender, tasty, and has high food value, rich in nutrition, with protein content accounting for 20% of the dry weight, containing 17 kinds of amino acids, a variety of vitamins, and inorganic salts, with health care effects, can regulate the physiological balance of the body, improve immunity. However, due to the serious degradation of Pleurotus tuoliensis cultivars, long production cycle, and low biological transformation rate, the cultivation efficiency is low, which affects the development of the industry and the progress of scientific research. Therefore, Lifeasible has launched Agrobacterium-mediated Pleurotus tuoliensis genetic transformation services to meet the research needs of researchers on Pleurotus tuoliensis, to establish a stable genetic transformation system, to screen and breed excellent varieties, and even to assist in variety development and marketing.

What We Offer

Lifeasible provides Pleurotus tuoliensis genetic transformation technology services, which as a strain improvement method can overcome the problems of traditional hybridization methods such as tedious and lower productivity, especially for traits and other problems that are difficult to solve by traditional hybridization methods.

Lifeasible provides several Agrobacterium strains such as LBA4404, GV3101, EHA105, AGL-1, etc. And we also provide several optional vectors including pCAMBIA1301, pCAMBIA1302, pCAMBIA1304, etc., all of which can be integrated into the host chromosome to build a stable Pleurotus tuoliensis genetic transformation system for you. Hygromycin is a widely used antimicrobial agent and has been used since 1992 for resistance transformation in Lentinula edodes, so we recommend you to choose Hygromycin (Hyg^R) resistance screening method in Pleurotus tuoliensis genetic transformation.

The use of homologous promoters not only facilitates the recognition of regulatory factors and reduces methylation, but also the use of homologous promoters not only facilitates the recognition of regulatory factors and reduces methylation, but also facilitates the recognition and binding of host cell RNA polymerase and the improvement of transformation efficiency. So Lifeasible recommends the use of the Pleurotus tuoliensis endogenous ras promoter to improve transformation efficiency.

Service Flow

The recombinant plasmid was transferred into Agrobacterium tumefaciens, and the target gene was transferred into Agrobacterium tumefaciens by Agrobacterium tumefaciens-mediated method using Pleurotus tuoliensis mycelium as the recipient material. This is a very complete and mature system, and our technical team has a lot of experience.

Why Choose Lifeasible

Lifeasible, as a leading plant technology company, provides Pleurotus tuoliensis genetic transformation technology services. Our professional team and stable genetic transformation system can assist our customers in strain selection, variety improvement, key gene function research, etc. With the attitude of providing the most cost-effective technical services to our clients, we invite you to supervise the whole project process, with a professional team at your service, we are always your best choice.