Volvariella volvacea Transformation

Volvariella volvacea is a saprophytic fungus native to tropical and subtropical regions with high temperatures and rainfall and is mostly grown on fiber-rich substrates. Volvariella volvacea are rich in many essential amino acids such as serine, alanine, and glycine. It also has high medicinal value and contains many vitamins, which can improve human health and reduce the absorption of cholesterol. Moreover, Volvariella volvacea can secrete cellulase, hemicellulase, ligninase, and other decomposition enzymes, and can grow on a variety of substrates containing cellulose and lignin, so it is widely cultivated and has a short production cycle. It is a class of edible mushrooms with high economic value and broad development prospects. However, because of its intolerance to low-temperature freezing, its mycelium will die by autolysis under conventional low-temperature freezing conditions, so genetic transformation breeding of Volvariella volvacea has been a hot research topic.

What We Offer

Lifeasible provides Agrobacbterium-mediated methods for transferring specific recombinant plasmids into Volvariella volvacea, and we have established an efficient genetic transformation system for Volvariella volvacea. In our platform, genes with improved agronomic traits can be transferred into the Volvariella volvacea to obtain normally expressed, genetically stable, and desirable strains. We have been providing professional services for research on Volvariella volvacea variety improvement and genome function through genetic engineering.

Figure 1. PCR analysis of afp gene in putative cold stress-resistant transformants of V.volvacea. (Wang J, et al. 2008)

We offer Agrobacterium strains such as EHA105 (Rif^R), EHA101 (Kan^R), LBA4404 (Rif^R), A281 (Rif^R, Nal^R), LBA4404, GV3101, etc. We screen for resistance with Hyg^R. We also offer plasmids with pCAMBIA 1301, pBGgHg, pLg-afp235 and other series. The commonly used reporter genes are GFP, GUS, and DsRed2. We also screen the AS content in advance for optimal concentration during pre-culture and co-culture. The reason for doing this is to match the optimal AS content for each experiment, and we also strictly control the co-culture temperature and time, the selection of Agrobacterium strains, and other influencing factors to ensure high transformation efficiency.

Service Flow

Why Choose Lifeasible

Lifeasible provides Agrobacterium-mediated genetic transformation services for Volvariella volvacea. With our well-established platform and rich experience, we provide our customers with the most cost-effective technical services to fully assist your research.

• We have a professional technical team that has been engaged in the genetic transformation of edible bacteria for many years, which can guarantee the success rate of the experiment.
• We have an efficient experimental process. We adopt the Agrobacterium transformation method, which is fast, efficient, stable, and single-copy insertion-based, because of the characteristics of Volvariella volvacea, we only support Hygromycin (Hyg^R) resistance screening.
• We have perfect service support. Professional technical consultation and guidance are provided in the front stage to optimize the project. Communicate and exchange at any time during the experiment to keep abreast of the progress. The agreed results will be provided according to the contract after the project is completed.