Nicotiana benthamiana Transformation

Nicana benthamiana belongs to the genus Tobacco in the Solanaceae (Solanaceae) family and is amphiploid with 38 chromosomes. Due to its unique properties and versatility, Nicana benthamiana has become a cornerstone of plant research. As a host for various plant pathogens (especially viruses), Nicana benthamiana provides an excellent system for studying plant-pathogen interactions. In addition, it has the advantages of a short life cycle, high transformation efficiency, and ease of genetic manipulation techniques. These attributes make Nicana benthamiana a valuable model plant for researchers in various disciplines, including genomics, biochemistry, metabolomics, and pathology. Many functional genomics tools are available for this species, including viral vectors, RNA interference (RNAi), ethyl methanesulfonate mutagenesis, Agrobacterium infiltration, protoplast transfection, and Agrobacterium-mediated stable transformation.

Fig. 1. Protoplast regeneration in N. benthamiana. (Hsu C T, et al, 2021)

Solutions

Lifeasible is an industry leader in the field of plant genetic transformation, offering a variety of solutions for Nicana benthamiana genetic transformation. Our experts are committed to developing simplified and efficient methods for introducing desired genetic modifications into this model plant species. Our services cover a range of innovative technologies and approaches that ensure reliable and reproducible transformation results.

• Agrobacterium-mediated Transformation
  We offer Agrobacterium-mediated Nicana benthamiana genetic transformation. This method utilizes the natural ability of Agrobacterium rhizogenes to transfer its DNA into plant cells. The main workflow uses leaf discs as explants, which are then co-cultured with Agrobacterium strains carrying the desired transgene. Subsequently, regenerating shoots from the explants are induced through organ differentiation.
  The advantages of this approach include the efficient introduction of the transgene, relatively fast turnaround time, and high transformation efficiency. Our protocol is optimized to ensure reproducibility and scalability, which enables the generation of a large number of transgenic events.
• Direct Delivery and Rapid Processing of Agrobacterium Co-cultures
  In addition, we offer rapid processing Agrobacterium co-culture to transform Nicana benthamiana. This method approach involves the provision of developmental regulators to Nicana benthamiana seedlings grown under sterile conditions that can induce the formation of abortive meristematic tissues leading to the development of buds and the subsequent transformation of Nicana benthamiana. This approach can provide rapid and robust transformation results.

To further enhance the transformation efficiency of Nicana benthamiana, we also have advanced vector systems customized specifically for this species. These vectors are carefully designed to optimize gene expression, improve transgene stability and ensure precise integration into the plant genome. By combining techniques such as Southern blot analysis, Northern blot analysis, and Western blot analysis, we ensure accurate detection and confirmation of the presence and expression of transgenes in transformed Nicana benthamiana plants.

Lifeasible is committed to providing cutting-edge genetic transformational services for Nicana benthamiana to advance science and drive innovation. We aim to introduce genetic modifications to Nicana benthamiana to help our clients study all aspects of plant biology, including gene function analysis, pathway elucidation, plant-pathogen interactions, crop improvement strategies, etc. If you are interested in our solutions, please contact us for technical consultation and quotation.