Detection of NO Signaling in Plant Cells

Nitric oxide (NO), as an important signaling molecule, regulates many developmental processes such as seed germination, root morphogenesis, and floral organogenesis and is involved in the regulation of stomatal movement as well as the response of plants to a variety of abiotic stresses and pathogen infestation. It has been shown that the arginine-dependent NOS pathway and nitrite-dependent NR pathway are the main enzymatic synthetic pathways for NO production in plant cells and that NO and its derivatives can directly modify the metal groups, cysteine, and tyrosine residues of substrate proteins and regulate the activity of target proteins and affect cGMP and Ca²⁺ through chemical modifications such as metal-nitrosation, mercapto-nitrosation, and Tyr-nitrosation. Through chemical modifications such as metal nitrosylation, mercapto-nitrosylation, and Tyr-nitrosylation, the activity of target proteins is regulated, and downstream signaling pathways such as cGMP and Ca²⁺ messenger system is affected to regulate corresponding physiological processes.

Lifeasible is a professional plant research company. Relying on the advanced technology platform and years of research experience, we are committed to the application of comprehensive technology to help researchers study the molecular mechanism of NO signaling more comprehensively and deeply in plant cells, which provides essential information for the understanding of related biological processes and plant improvement.

Role of nitric oxide in plant cells

NO acts as a signaling molecule and plays an essential regulatory role in plant growth and development, such as plant responses to biotic and abiotic stresses, programmed cell death (PCD), respiration, photomorphogenesis, fruit ripening, leaf extension, stomatal closure, senescence, seed germination, flowering regulation, root development, and hormonal responses, among other plant growth and developmental processes. NO signaling plays a vital role in the activation or augmentation of plant defense responses. NO signaling plays an important role in activating or enhancing plant defense responses.

What do we offer?

We provide a variety of advanced technical support in plant cell NO signaling research, which plays an important role in helping researchers involved gain a deeper understanding of the mechanisms of NO production, delivery, and response in plant cells; at present, some of the most applied techniques in plant cell NO signaling research are as follows.

• Fluorescent probe technology. We detect intracellular NO levels using NO-specific fluorescent indicators such as 4,5-diaminofluorescein diacetate (DAF-2DA) or DAF-FM DA. These indicators fluoresce upon reaction with NO, and NO levels can be quantified by fluorescence microscopy or flow cytometry.
• Electrochemical probes. We utilize NO-sensitive electrodes or electrochemical sensors to monitor the dynamics of NO in cells by measuring the change in current between the electrode and NO. Electrochemical probes are useful for monitoring NO release and concentration changes in real time.
• Gene expression analysis. We can study NO-induced changes in gene expression by real-time fluorescence quantitative PCR. This helps to identify genes that respond to NO signaling and to understand the regulatory role of NO in plant physiological processes.
• Mass spectrometry. Mass spectrometry occupies an essential position in the study of NO signaling in plant cells, and we utilize mass spectrometry techniques, such as mass spectrometry-mass spectrometry (MS/MS), which allows us to quantify the concentration of NO and its related products. This contributes to an in-depth understanding of NO metabolic pathways and catabolic products.

In addition, in NO signaling research, we use yeast two-hybrid technology, cellular immunoprecipitation, etc., to study protein interactions in the NO signaling pathway, which is used to reveal the functions and regulatory mechanisms of key proteins in the NO signaling pathway.

Advantages of our services

Lifeasible is committed to plant cell biology research, we aim to provide more comprehensive research services related to plant cell signaling by utilizing advanced technical methods and professional cell biology knowledge. The comprehensive technical support we provide is of great significance in helping researchers to advance the field of cell signaling research, and provides more possibilities to further deepen people's understanding of plant nitric oxide signaling network and to promote the application of related fields. If you are interested in us, please feel free to contact us.