Detection of Plant Genetic Variation

Crop improvement has been the basis for food security for a growing population. Plant breeding works by exploiting genetic variation among individuals within a plant species and combining desired characteristics into new and improved varieties. Genetic variation is the variation in gene sequences between individuals in a plant population. Allelic variation is the basis of genetic variation and is expressed through different phenotypes. Processes such as mutation, random mating and fertilization, and gene duplication may introduce new genes and alleles that increase genetic variation. Genetic variation provides the basis for plant genetic diversity and reflects the degree/amount of variation present within a population. Without genetic variation, populations would not be able to adapt to different climatic conditions and could easily become extinct. In short, genetic variation is at the root of natural selection and is a crucial driver of the evolution of organisms. At the same time, the estimation of the extent and pattern of genetic variation is a prerequisite for the production of superior varieties. Lifeasible provides comprehensive plant genetic variation testing services to help you develop high-quality crop varieties for clients worldwide.

Fig. 1. Different sources of genetic diversity and their potential utilization in developing new crop varieties. (Salgotra R K et al., 2023)

Our Solutions

Genetic variation in plants is extensive and is a key factor in the adaptation of plants to different climates. There are many other methods used to amplify plant genetic variation, such as chemical or physical treatments, translocation breeding, synthetic hexaploidy, CRISPR/Cas9, etc. As a specialist in plant genetic engineering, Lifeasible provides professional solutions for detecting plant genetic variation, accurately detecting and analyzing both natural and epigenetic variation in plants. Our strict quality policy ensures the reliability of our results.

We offer various methods to detect the degree of similarity and difference between plant germplasm.

Morphological Markers

With high-throughput phenomics methods, we can provide detailed documentation of plant morphological traits, including characteristics from nutrient growth and reproductive growth to maturity stage. We ensure comprehensive and accurate data collection. In addition, we help you analyze complex traits such as yield and disease resistance by analyzing high-quality phenotypic data. This approach is easy, simple, inexpensive, and directly measurable.

Cytological Markers

By selecting markers related to morphological changes in chromosome size, shape, number, length, arm ratio, volume, cell division behavior, and DNA content. We offer Fluorescence In Situ Hybridization (FISH) and Genomic In Situ Hybridization (GISH) techniques to characterize foreign gene infiltration in plant species and to dissect the genetic composition of natural and artificial hybrids.

Biochemical Markers

We offer isozyme marker technology to analyze and detect variation between and within plant species. This method is simple, fast, and inexpensive, making it ideal for projects that require low-level detection of genetic variation.

Molecular Markers

We offer a variety of molecular marker methods based on DNA sequence polymorphisms to detect plant genetic variation. Each marker system has its own advantages and disadvantages, and we will select the best solution for you. This approach offers the following advantages:

• Any small sample of plant material is possible.
• Genetically simple and highly reproducible.
• Widely distributed, stable, and highly polymorphic in the plant genome.
• Not dependent on plant stage and minimally affected by the environment.

Next Generation Sequencing Technologies

We offer a variety of sequencing technologies to comprehensively analyze plant genetic diversity and quickly identify useful genes and/or alleles.

• Whole genome resequencing
• Whole exome capture sequencing
• RNA sequencing (RNA-seq)
• Restriction site-associated DNA sequencing
• Sequencing genotyping (GBS)
• Amplicon sequencing

Applications of Plant Genetic Variation Detection

• Crop improvement
• Biodiversity conservation
• Climate adaptive agriculture

Lifeasible provides comprehensive content of plant genetic variation detection and can design customized solutions according to your specific requirements. We hope that our products and technology can satisfy our customers. We look forward to your inquiry. For more information, please contact us.