Detection of Plant Nematodes by Microfluidic PCR Technique

Polymerase chain reaction (PCR) mainly depends on the temperature, which is performed with repeated thermal cycling by providing the required temperatures for different reaction stages. Microfluidics provides a potential solution for increasing PCR speed, which can perform various PCR-based techniques, such as conventional PCR, RT-PCR, and qPCR. The principle of this technology is appropriately manufacturing small or complex PCR reactors to perform PCR at ultra-high heating rates.

Lifeasible provides services to customers worldwide covering the detection of plant nematodes by microfluidic PCR technique, including microfluidic digital PCR, optical microfluidic PCR, and single-cell microfluidic PCR. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Detection of Plant Nematodes by Microfluidic Digital PCR

• Digital PCR is a platform that uses a single nucleic acid template to perform PCR in an ultra-small volume such as a droplet, which facilitates absolute quantitative analysis compared with traditional PCR.
• Microfluidic digital PCR, which uses droplets to digitalize the PCR mix, may be effective for plant nematode detection over microfluidic PCR approaches. This approach is based on the qPCR principle.

Fig.1 A brief description of microfluidic RT-PCR assay.

• Lifeasible provides the detection of plant nematodes with microfluidic digital PCR. The sample is added to 1,000 individual bioreactors, each executing its amplification reactions. The development of this technology has expanded the number of PCR reactions that can be performed, with either a significant number of samples being examined for fewer targets or a smaller number of samples being checked for a more substantial number of targets.

Detection of Plant Nematodes by Optical Microfluidic PCR

• Optical systems present the advantage of not to induce any thermal mass and can thus accelerate thermal transitions, such as infrared lamps, lasers and so on.

Fig.2 Laser heating of PCR droplets film.

• We provide detection of plant nematodes with optical microfluidic PCR, which uses droplets as different bioreactors.

Detection of Plant Nematodes by Single-Cell Microfluidic PCR

• Single-cell microfluidic PCR technology uses fluid to separate single cells.
• We provide detection of plant nematodes with single-cell microfluidic PCR, which has the advantage of precisely quantifying and discriminating the genetic expression profile of every single cell from a sparse population of cells without loss of data due to the average of the gene expression.

Lifeasible has long-term devoted to the development and application of plant science. We are pleased to use our extensive experience and advanced platform to offer satisfied service and qualified products to satisfy each demand from our customers. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.