Detection of Plant Nematodes by Molecular Markers

A molecular marker is defined as any DNA sequence that shows polymorphism and can be detected using molecular techniques. Due to their stability, cost-effectiveness, and ease of use, many molecular marker techniques have been developed and utilized in different systems worldwide. Only a few of these techniques have gained global acceptance. However, it is an effective tool for analyzing the genetic variation of plant parasitic nematode populations between species and under species.

Lifeasible provides the detection of plant nematodes by molecular markers to assist our customers in nematode identification. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Detection of Plant Nematodes by RFLP

• The restriction fragment length polymorphism (RFLP) method can distinguish different species of plant nematodes because the DNA sequence structure of various species is different, and the recognition sites and the number of endonucleases are different. After restriction enzymes of different kinds are digested, the gel electrophoresis pattern will inevitably reflect the differences among species.
• Lifeasible provides detection of plant nematodes with RFLP, helping our customers explore various endonucleases to distinguish different nematode species. We also explore creative identification for plant nematodes by combining them with other molecular markers.

Detection of Plant Nematodes by RAPD

• Usually, in the genome of a species, there are about 10 inverted repeats in every 2000 bp long sequence.
• The primer of random amplified polymorphic DNA (RAPD) is a 10 bp oligonucleotide single-strand random primer, and different random primers can be used to detect sites covering the whole genome, thus reflecting the real diversity of this species.
• We provide detection of plant nematodes with RAPD, helping our customers develop multiple nematode random primers and/or their combinations for nematode detection.

Detection of Plant Nematodes by SCAR

Fig.1 The process of detecting plant nematodes by SCAR.

• Sequence characterized amplified region (SCAR) technology is a new molecular marker based on RAPD technology. A pair of 24-base complementary primers are designed on the species-specific RAPD sequence, and the original template DNA was amplified. The amplified region of the characteristic sequence was detected by amplification.
• We provide detection of plant nematodes with SCAR, helping our clients test the sensitivity of the marker detection system. This method can quickly detect a large number of individuals, and the results are stable and reproducible.

Lifeasible has a long-term commitment to the development and application of plant nematodes. We are pleased to use our extensive experience and advanced platform to provide satisfactory service and qualified products to meet the needs of our customers. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.