Determination of Hen's Egg White Lysozyme Content

Lifeasible specializes in milk- and milk product-related testing and offers services to help determine the content of egg white lysozyme that may be added to milk and milk products.

Egg white lysozyme is a protein found in egg whites that has antimicrobial properties and lysis the cell wall of some bacteria. Egg white lysozyme is approximately 3-4% of the egg white. Lysozyme is also present in hen's saliva and in tear liquid. Egg white lysozyme can be used as a natural preservative in food and beverage products. Measuring egg white lysozyme in milk and cheese can be used to determine the presence of egg white in these products. Additionally, measuring egg white lysozyme can be used to monitor the effectiveness of food safety protocols. Lifeasible offers a service to determine egg white lysozyme in accordance with international standards.

Fig. 1 The tertiary structure of egg white lysozyme from chicken eggs (Silvetti et al., 2017).

Our Services

We help detect egg white lysozyme using high-performance liquid chromatography (HPLC). The method applies to milk and cheese samples.

The method requires the precipitation of casein and denatured whey proteins from milk and cheese samples first. This is followed by determining acid-soluble hen's egg white lysozyme using reversed-phase HPLC and fluorescence detection. The method can also be combined with a mass spectrometer (MS) to verify hen's egg white lysozyme.

Operation flow:

Main reference standard:
ISO 27105:2016

Note

This method is suitable for determining low levels of hen's egg white lysozyme, with a limit quantification of 10 mg/kg. If you know that hen's egg white lysozyme is high in your sample, please let us know so we can dilute the test sample and then measure it.

Lifeasible helps to determine the hen's egg white lysozyme content in milk and cheese samples with reference to the latest international standards. With our excellent expertise and professional equipment, we are a reliable partner for determining hen's egg white lysozyme content. Please cooperate with us!