Determination of Sucrose Content

Sucrose is an important component of sweetened condensed milk. Lifeasible helps to determine the sucrose content in sweetened condensed milk to help confirm the quality.

It is essential to determine the sucrose content in sweetened condensed milk (Fig.1). Sweetened condensed milk is a traditional dairy product made from concentrated milk and sucrose. It contains 60% sugar in its water phase and has a preservative effect and a long shelf life. However, excessive consumption of sucrose may be harmful to consumers' health, such as causing tooth decay. The use of trehalose and isomaltulose is being considered as an alternative to sucrose in sweetened condensed milk for the prevention of dental caries. Lifeasible provides professional and reliable milk-related testing services and provides service to help determine the sucrose content in sweetened condensed milk.

Fig. 1 sweetened condensed milk.

Our Services

Here, we help to determine the sucrose content in sweetened condensed milk based on the polarimetric method. We can help determine sweetened condensed milk prepared from whole, partially skimmed, or skimmed milk, and sucrose. The prerequisite for this determination is that the sweetened condensed milk contains only sucrose, and no other altered sucrose is added.

Determination principle:

Lactose is the most abundant sugar in milk. The method requires the removal of the effects of the mutarotation of lactose with ammonium hydroxide. The sample is subsequently neutralized and clarified using zinc acetate and potassium hexacyanoferrate (II) to remove proteins. Then the optical rotation of the filtrate and the inverted filtrate obtained after sucrose hydrolysis is determined. Sucrose content is calculated from the change in optical rotation on inversion.

Operation flow:

Main reference standard:
ISO 2911:2004

Lifeasible offers services to help determine the sucrose in sweetened condensed milk. We recommend this method when it is known that only sucrose is added to sweetened condensed milk. If other sugars are added, we offer high-performance anion exchange chromatography combined with the pulsed amperometric detection method to help determine. Please contact us to discuss the determination of sucrose.