Development of Cytoplasmic Polyhedrosis Virus (CPV) Insecticide

Development of Cytoplasmic Polyhedrosis Virus (CPV) Insecticide

Cytoplasmic Polyhedrosis Virus (CPV) is a natural pathogen of many insect species. The usual site of CPV infection of target insects is the midgut tissue. This is where the larvae lose appetite, move slowly, have a shrunken carapace, and discharge polyhedral mucus from the anus.

Lifeasible is committed to providing comprehensive and effective solutions for biopesticide development. We continue insect virus research. We have developed a virus insecticide, which has been effective in agriculture and forestry pest control and achieved better control results.

Ultrastructure of Cytoplasmic Polyhedrosis VirusFigure 1. Ultrastructure of Cytoplasmic Polyhedrosis Virus (Vávra J, et al., 2016).

Application of CPV insecticide development

Plasmopolyhedrosis virus is easy to proliferate, has high virus yield, strong virulence, and broad insecticidal spectrum, and the resulting insecticide is highly effective and harmless to humans and animals, does not harm natural enemies. CPV has a wide range of hosts and can cause disease in a variety of insects, so it has a wide application prospect as a biocide. Our developed CPV insecticide is currently effective against pests in forests, vegetables, cotton, tea, and fruit trees. Red pine caterpillars, pine banding moths, and pink stripe night moths, for example, have been effectively controlled.

Specifications of the CPV insecticide's action

The following describe the actions of CPV insecticide. High specificity. A virus is harmless to insects that are not its target and can only parasitize one insect or population nearby. Under normal circumstances and away from direct sunlight, it is comparatively stable and may be stored for years without going inactive. It is safe for humans, animals, birds, beneficial insects, fish, etc., and has not evolved a pest resistance.

What do we offer for CPV insecticide development?

  • Virus detection and inspection. We do a genomic analysis of viruses using several molecular biology techniques, such as PCR and DNA sequencing, to ascertain their categorization and characterization to screen for virus strains with strong insecticidal activity.
  • Virus culture and production. To provide enough viral particles, we use cell culture methods like insect cell culture or other host cell culture systems. To increase virus yield and purity, we have also created the proper virus-growing media and conditions.
  • Viral activity assay. Determining the activity of CPV formulations is a step in the development process of CPV insecticides. Using molecular biology techniques, we can determine the virus' killing effect on host insects, allowing us to detect virus activity.
  • Virus strain improvement. We use genetic engineering techniques that can be applied to improve the virulence and insecticidal efficacy of CPV. This technique involves the insertion of specific genes into the virus genome to enhance its insecticidal ability and broaden its host range.

Our service workflow

Ultrastructure of Cytoplasmic Polyhedrosis Virus

Lifeasible aims to provide a comprehensive solution for developing efficient and sustainable biology. If you are interested in us, please feel free to contact us.

References

  1. Vávra J, et al. Occurrence, pathology, and ultrastructure of iridovirus and cytoplasmic polyhedrosis viruses in daphnids from the Czech Republic. J Invertebr Pathol. 2016; 140:35-38.
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