Development of Nuclear Nuclear Polyhedrosis Virus (NPV) Insecticide

NPV is a class of specialized insect viruses whose processed formulations are viral insecticides that provide excellent control of single-target Lepidopteran pests. NPV mostly develops in the nucleus of the host's blood, fat, skin, limbs, etc. NPV enters the insect via the mouth and is digested by gastric juice. This frees rod-shaped virus particles that enter the body lumen through the midgut epithelium, invade the cell, proliferate in the nucleus, and later invade. The virus invades healthy cells until the insect is killed.

Lifeasible has developed insect and viral insecticides that provide effective pest control guidance in agriculture and forestry. Our NPV insecticide development solutions are vital in advancing biological control sustainability.

Characteristics of NPV insecticides

• A virus can only parasitize one type of insect or a species that is close by, even though NPV has a broad host range and primarily parasitizes Lepidoptera.
• Long action duration. NPV has a significant and lasting impact. The virus can suppress insect populations for longer periods because it can remain in the environment for several years after being released.
• Ecologically safe. NPV pesticides may be used frequently in the field, are safe and non-toxic, and do not develop pest resistance.

What technical support do we provide for NPV insecticide development?

NPV is an insect virus widely used for biological control. The development of nucleopolyhedrosis viruses as insecticides can utilize the following biotechnologies.

• Virus isolation and identification. We isolate NPV strains with insecticidal activity from the natural environment and identify and classify them.
• Virus multiplication and purification. We use insect cell culture techniques to inoculate NPV into suitable host insect cells to induce virus proliferation. Pure virus particles were isolated from cells by virus particle purification techniques.
• Viral genome sequencing. By sequencing NPV's genome, we can understand its genome structure, encoded proteins, and related functions, providing the basis for subsequent studies.
• Genetic engineering and genome editing. We use genetic engineering techniques to modify and edit NPV's genome to alter its insecticidal properties, enhance its virulence, or extend its host range.
• Fermentation techniques. We inoculate NPV strains into large-scale fermenters and use fermentation techniques to multiply the virus. This can be achieved by optimizing the medium composition, temperature, pH, and other conditions.

Advantages of our services

Lifeasible has many years of experience in research services in the biological field. We utilize advanced biotechnology to provide effective solutions for biopesticide development. We are at the forefront of scientific advances and are committed to providing various effective pest management solutions for biopesticide development using the latest technologies. If you are interested in us, please feel free to contact us.