Double-stranded RNA Bacterial Expression

Double-stranded RNA is expressed by bacteria using modified bacterial strains to create particular dsRNA. When the target pest touches the dsRNA produced by the modified bacteria, it will trigger RNA interference mechanism that blocks the expression of critical genes in the pest, ultimately resulting in pest death.

Lifeasible specializes in life sciences and biotechnology development. Our dsRNA bacterial expression provides a greener, more environmentally friendly, and more efficient solution for agricultural and forestry pest control. We construct specific expression vectors and transfer them into bacteria to express dsRNA directly in the body of the bacteria. We then purify the dsRNA through a purification step or inactivate the bacteria directly for RNAi experiments. We have constructed various dsRNA expression platforms for bacteria and aim to provide one-stop solutions according to customers' needs.

Advantages of dsRNA bacterial expression

• High yield. The bacterial expression system we offer can produce large quantities of dsRNA, suitable for large-scale control applications of targeted pests.
• Target specificity. By designing double-stranded RNA sequences complementary to target pest genes, we can precisely control RNAi effects to minimize damage to non-targeted target organisms.
• Ecological safety. Unlike chemical insecticides, dsRNA-based approaches are environmentally friendly and characterized by green and non-polluting effects.
• Reducing pest resistance. RNAi-based strategies have the potential to delay or prevent insect resistance and can be used repeatedly or for extended periods in the field.
• Low-cost. Once a bacterial strain has been engineered to produce the desired dsRNA, it can be mass-produced at a relatively low cost. Cost-effectiveness is particularly beneficial for large-scale pest control efforts.

Process of double-stranded RNA (dsRNA) bacterial expression

The process of dsRNA bacterial expression involves introducing exogenous dsRNA into a bacterium and enabling it to express that RNA, its expression generally proceeds as follows.

• Identification of target genes. Finding target genes in pest species is the initial stage in the dsRNA bacterial expression process. These genes typically play a role in fundamental physiological functions, including growth, metabolism, or reproduction, that are crucial for pest survival.
• Designing the dsRNA molecule. After identifying the target gene, dsRNA molecules are designed to match specific regions of the gene's mRNA sequence.
• Cloning of dsRNA expression constructs. The designed dsRNA sequence is cloned into plasmids suitable for bacterial expression. These plasmids contain strong bacterial promoters that drive dsRNA expression. In addition, marker genes are often included to facilitate the selection of successfully transformed bacteria.
• Transformation of bacteria. Transformation methods like electroporation or heat shock are used to introduce expression constructs into a bacterial host, such as E. coli. employing indicators of antibiotic resistance to choose microorganisms that have effectively undergone transformation.
• Fermentation and dsRNA production. Transformed bacteria to produce large amounts of dsRNA. As the bacteria multiply, they express dsRNA, and it accumulates within the bacterial cells.
• Extraction. When sufficient amounts of dsRNA have been produced, bacterial cultures can be harvested and processed to extract the dsRNA.

Our services workflow

Our technical advantages

• High efficiency. We have a wide range of expression vectors and strains, which can greatly improve dsRNA induction efficiency and dose.
• Specialization. We provide vector construction of any gene fragment in the length range of 100 bp to 4000 bp, which can maximize customers' project requirements satisfaction.
• One-stop. We provide customers with one-stop solutions for project communication, gene cloning, vector construction, strain transformation, etc. We help customers save time and energy.

Lifeasible has many years of research experience in insect pest control. We provide dsRNA bacterial expression, which provides an innovative and effective solution for biological pest control. At present, dsRNA-based bacterial expression has been effectively used to control various agricultural and forestry pests and achieved good results. If you are interested in us, please feel free to contact us.