Duckweed Transformation

Duckweeds are flowering aquatic plants, comprising five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia), widely distributed in freshwater habitats worldwide. Their small size, morphological simplicity, and rapid growth (mainly asexual reproduction and floating growth) make them attractive models for understanding basic plant biological processes. In addition, Duckweeds have a high rate of starch accumulation, low lignin content, and a high capacity for absorbing pollution. They can be an excellent candidate for biofuel production and widely used for wastewater bioremediation. Even, duckweed is an ideal plant system for producing recombinant proteins due to its high protein content. The emergence of tools such as CRISPR/Cas9-mediated genome editing has made it possible to efficiently genetically transform duckweed by knocking out or knocking in the genes of interest in the duckweed genome in a short period of time.

Fig. 1. Stable, efficient genetic transformation of Lemna aequinoctialis. (Liu Y, et al, 2019)

Solutions

Lifeasible is an industry leader in plant genetic transformation and is dedicated to providing comprehensive genetic transformation services for a wide range of duckweed species across five genera. Our state-of-the-art facilities and highly skilled team enable us to provide efficient and reliable transformation programs that ensure accurate gene integration and expression. We offer customized approaches for different duckweed species and are committed to delivering results that meet the unique requirements of each project.

Our team of experts works closely with our clients to understand their specific research goals and design customized experiments. We take into account the species of duckweed, as well as through careful optimization of factors such as Agrobacterium strains, exosome types, co-culture conditions, and selection markers; we provide you with effectively optimized protocols for duckweed Agrobacterium-mediated genetic transformation, including but not limited to:

• Lemna gibba Genetic Transformation
• Lemna minorGenetic Transformation
• Lemna turionifera Genetic Transformation
• Landoltia punctata Genetic Transformation
• Spirodela polyrhiza Genetic Transformation
• Wolffia arrhiza Genetic Transformation
• Wolffia globosa Genetic Transformation

In addition to Agrobacterium-mediated transformation, we also offer particle bombardment transformation services for duckweed. Our skilled technicians utilize advanced particle bombardment technology to ensure precise and efficient delivery of genetic material into duckweed cells, thus expanding the possibilities for genetic manipulation and functional studies. This method allows direct gene transfer without the need for Agrobacterium infection.

• Selection and Regeneration
  We use various selection markers, such as antibiotic resistance genes or reporter genes, to identify and isolate transformed cells. Our protocol ensures rigorous selection of transformed cells, minimizes false positives, and enables the production of stable transgenic lines. After selection, we optimize regeneration conditions to promote the development of healthy, robust transgenic plants.
• Gene Expression Analysis
  We provide molecular characterization of transgenic duckweed, such as PCR, Southern blotting, and gene expression analysis using quantitative real-time PCR or other suitable methods. We provide detailed reports summarizing experimental procedures, results, and data interpretation.

Applications of Duckweed Transformation

• Understanding the development and physiology of duckweed species
• Enhance the ability of duckweed to remove pollutants from water sources or soil.
• Improve traits such as biomass production, nutrient content, or tolerance to biotic and abiotic stresses in duckweed.

Lifeasible is committed to providing high-quality genetic transformation services for duckweed. Through our expertise in experimental design, optimization protocols, and comprehensive analysis, we support scientific progress and contribute to sustainable solutions in agriculture, environmental remediation, and bioenergy. Contact us today to explore our Duckweed Genetic Transformation Services to accelerate your research or move your industrial applications forward.