Eukaryotic transcriptome sequencing is a method to comprehensively analyze all RNA molecules in a cell through high-throughput sequencing technology, which can reveal key information such as gene expression levels, variable splicing, new transcripts, and SNP variants. In agricultural research, this technology provides an important basis at the molecular level for analyzing crop resistance mechanisms, optimizing molecular breeding, and regulating metabolic pathways.

Lifeasible is committed to the forefront of agricultural research. We have established an advanced eukaryotic reference transcriptome sequencing (RNA-seq) technology platform, which can sequence all the mRNAs transcribed in a specific eukaryotic tissue or cell in a particular state, and then compare with the reference genome, which allows us to comprehensively and rapidly analyze the sequence and abundance information of the mRNAs, and analyze the structure of the genes and new transcripts generated. The mRNA sequence and abundance information can be analyzed quickly and comprehensively, and the gene structure and new transcripts generated can also be analyzed.

Explore Our Eukaryotic Transcriptome Sequencing

• Sample preparation. We accept many types of samples, including cells, animal tissues, plant tissues, and so on. Our professional team will select the appropriate RNA extraction method according to the sample type and research purpose to ensure that the quality and purity of the extracted RNA meet the requirements of subsequent experiments.
• Sequencing. We use advanced sequencing platforms. For species with reference genomes, we provide sequencing services with reference transcriptomes. For species without reference genomes, we provide sequencing services without reference transcriptomes, obtaining transcript information through de novo assembly.
• Data analysis. Our bioinformatics team has rich experience in data analysis, using a series of advanced analysis tools and algorithms to provide you with comprehensive and in-depth data analysis services. This includes but is not limited to raw data quality control, transcript assembly and annotation, gene expression quantification, differential expression gene analysis, etc.
• Personalized service. We can provide personalized service solutions according to customer's project needs. Customers can choose different sequencing depths, data analysis contents, etc., according to their research objectives.

Application of Eukaryotic Transcriptome Sequencing

• Crop resistance studies. Dissecting gene expression regulatory networks under drought, salinity, and other stresses.
• Molecular breeding optimization. Screening key genes and molecular markers related to yield and disease resistance.
• Metabolic pathway analysis. Reveal the regulation mechanism of the secondary metabolite synthesis pathway (e.g., flavonoids, alkaloids).
• Functional genomics. Mining the role of new genes and non-coding RNAs in growth and development.

Our Service Workflow

• Sample preparation. We perform rigorous quality testing of samples, including RNA integrity and purity, to ensure that the quality meets the requirements and to select appropriate RNA extraction methods.
• Library construction. After the extracted RNA has been quality tested, we will proceed to library construction.
• Sequencing. After the constructed library is quality tested, we will select the appropriate sequencing platform and sequencing strategy for sequencing according to your research needs and sample characteristics.
• Data analysis. We can provide basic and advanced data analysis.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Eukaryotic Transcriptome Sequencing

• Fast and stable. Based on our premium sequencing platform, we ensure fast and stable sequencing data analysis and delivery.
• Professional program design. We adopt appropriate extraction methods for different species and tissue parts and provide different library construction methods according to the different needs of customers.
• Experienced project service. We have successfully sequenced eukaryotic reference transcriptomes of thousands of species with reference genomes, such as rice, Arabidopsis thaliana, tomato, peanut, cotton, etc., and have rich experience in project services.

Lifeasible is committed to providing efficient, accurate, and comprehensive eukaryotic transcriptome sequencing services and professional bioinformatics analysis to help you make breakthroughs in your research. If you are interested, please feel free to contact us.