Fruit Antioxidant-Related Metabolites Determination

Antioxidant-related metabolites play an important role in affecting the freshness and flavor of fruits, and the accurate determination of antioxidant-related metabolites in fruits plays a vital role in promoting the research on the quality and nutritional value of fruits as well as ensuring the market value of fruits. Relying on the advanced technology platform and the experienced research experience, Lifeasible is dedicated to comprehensively determining the metabolites of fruits, especially the antioxidant-related metabolites.

Importance of Fruit Antioxidant-Related Metabolites Determination

• Nutrition. Determination of common antioxidant metabolites in fruits, such as vitamin C, flavonoids, phenolic compounds, and carotenoids, provides various benefits to human health. By detecting antioxidant-related metabolites in fruits, theoretical support can be provided for fruit nutrition research.
• Freshness preservation. During fruit ripening, harvesting, and storage, oxidative stress leads to an increase in reactive oxygen species in fruits, which triggers a series of reactions, such as pigment decomposition and nutrient loss, affecting the color, texture, and nutritional value of fruits. The measurement of antioxidant metabolites in fruits can help researchers analyze this process and apply it to fruit quality improvement to slow down the changes in fruit quality caused by oxidative stress.
• Economic value. The content of antioxidant metabolites in fruits is closely related to the market value of the fruits, and antioxidant-rich fruits have good taste and long shelf-life, which largely affects the economic value of the fruits.

Our Service for Fruit Antioxidant-Related Metabolites Determination

We provide analysis services for fruit antioxidant metabolites, including vitamin C, polyphenols, carotenoids, flavonoids, etc.

Vitamin C

Vitamin C, a water-soluble antioxidant that directly scavenges ROS and protects against oxidative stress damage, is widely found in citrus, strawberries, kiwis, and other fruits. We offer a variety of methods for determining vitamin content in fruits; the two most commonly used methods are listed below.

• Titration. We commonly use iodine titration to determine vitamin C. The reducing properties of vitamin C are used to reduce iodine, and the endpoint of the reaction is determined by the change in color of the indicator.
• High-performance liquid chromatography (HPLC). HPLC is an accurate method for determining Vitamin C in fruits. It is characterized by high sensitivity and selectivity, making it more suitable for multi-sample, high-throughput assays.

Polyphenols

Polyphenol compounds are a class of antioxidants widely found in fruits, most commonly in apples, grapes, blueberries, and other fruits. In determining polyphenolic compounds in fruits, we most commonly use two methods.

• Folin-phenol reagent method. A blue color compound is produced by the reaction of a forint-phenol reagent with polyphenolic compounds, which is quantitatively analyzed using spectrophotometry.
• High-performance liquid chromatography (HPLC). HPLC has shown good application in detecting specific polyphenol chemicals in complex fruit extracts.

Carotenoids

Carotenoids are a group of fat-soluble antioxidants that inhibit the production of free radicals to reduce cellular damage caused by oxidative stress. Carotenoids, lutein, and lycopene are common carotenoids found mainly in fruits such as carrots, tomatoes, sweet potatoes, and persimmons. In carotenoid determination, we take advantage of the fact that carotenoids have light-absorbing properties at specific wavelengths. We use UV spectrophotometry to quantitatively determine their content, separate and quantitatively analyze multiple carotenoids in fruits by HPLC.

Fig.2 Process of our services. (Lifeasible)

Why Choose Us?

• Professional and technical team. We have experienced technicians and advanced laboratory equipment, including HPLC, UV spectrophotometry, etc., to ensure professional and efficient completion of testing.
• Comprehensive testing services. We provide one-stop service from sample processing to data analysis, including customized experimental plan, raw data and experimental report.
• A wide range of assays. We cover a wide range of indicators related to oxidative stress and other research areas including vitamin C, flavonoids, carotenoids, polyphenols, etc., to meet the needs of our clients under different fruits and research objectives.
• Scientifically correct results. We guarantee accurate, objective and credible test results, providing detailed experimental methods and related graphs of the results.

Lifeasible is committed to providing efficient and accurate measurements of antioxidant compounds in fruits to support fruit quality-related research. If you are interested, please feel free to contact us.