Functional Analysis of Plant Endoplasmic Reticulum Proteins

The endoplasmic reticulum (ER) is a dynamic subcellular compartment that controls secretory pathways at multiple checkpoints in protein biosynthesis, including folding, quality control, signaling, and degradation. In addition, ER plays an important role in abiotic and biotic stress resistance through unfolded protein response signals. The ER is also an essential cellular compartment for calcium storage and carbohydrate metabolism.

Lifeasible is a leading provider of comprehensive, high-quality services for the analysis of plant endoplasmic reticulum proteins. We hope to partner with you to explore new frontiers in plant science and accelerate your discovery.

Functional Analysis of KMS1 and KMS2 Proteins in Plant Endoplasmic Reticulum

• Secretory proteins are produced by ER-bound ribosomes as peptide precursors and complete proper folding in the ER cavity before export. KMS1 and KMS2 are genes involved in the early secretory pathway and Golgi /ER organization and are conserved in eukaryotes.
• It is reported that KMS1 and KMS2 proteins play a role in maintaining ER / Golgi structure and protein secretion.

Fig.1. The endoplasmic reticulum network contract as performed of KMS2 RNAi in Arabidopsis hypocotyls. (Wang P, et al., 2011)

• Lifeasible provides a protocol for functional analysis of KMS1 and KMS2 proteins. The services include confocal microscopy analysis of protein localization in plant cells, demonstrating the distribution of the proteins in the ER.
• We provide osmotic OD and confocal microscopy, which verify the involvement of C- or N-terminal lysine motifs in ER retrieval.
• In addition, we help observe the morphology of ER through the overexpression or knocking down of KMS1 and KMS2.

Functional Analysis of Calnexin Proteins in Plant Endoplasmic Reticulum

• Calnexin is an abundant type I membrane protein first described in mammalian ER. In plants, a calnexin-like protein is first cloned in Arabidopsis.
• Calnexin is a well-known ER chaperone that transiently binds nascent glycoproteins. In addition, it is used as a model to define the signals and mechanisms whereby a type I membrane protein is retained in the plant ER.
• From the above points, we provide a protocol for the functional analysis of calnexin proteins. To determine where calnexin proteins are expressed, plants are engineered to express GUS under the control of calnexin promoters. The transcript abundances of proteins associated with the ER are examined by western blot.

Lifeasible provides analysis of plant endoplasmic reticulum to our clients worldwide. With years of experience in biological services, our advanced platforms can help our clients solve various difficulties in detecting glutathione with plant research. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.