The diameter of KDEL vesicle (KV) is between 200 nm and 500 nm. After budding from the endoplasmic reticulum (ER), KV reaches the vacuole directly without passing through the Golgi. In plants, KV formation and vacuolar transport through KV are associated with the KDEL sequence of cysteine protease with KDEL signal. Most soluble ER resident proteins contain the KDEL or HDEL 4 peptide sequence at the C-terminus, called ER resident signal. The Golgi apparatus recognizes this signal and sends the resident proteins that have escaped back to the ER. Besides, the H / KDEL system is highly conserved in plants.
Lifeasible develops an advanced platform equipped with advanced instruments and professional staff for functional analysis of KDEL vesicles with a high standard. We customize featured services according to the customers' demand.
Fig.1. Electron photographs showing that KV is immunogold labeled with antibody to KDEL sequence. (Toyooka K, et al., 2000)
Fig.2. Intracellular localization of SP-GFP-SHEP or SP-GFP-SHEPΔKDEL in tobacco BY-2 cells. (Okamoto T, et al., 2003)
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References
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